ArticlePloS one2017
Identification of odorant binding proteins and chemosensory proteins in Microplitis mediator as well as functional characterization of chemosensory protein 3.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- The Chemoreceptive Molecular Mechanism Underlying CSP-Mediated Recognition of Seed Elaiosome fromGenes · 2025Article
- Cloning, Expression, and Odorant Binding Characteristics of the Chemosensory Protein 1 in Asecodes hispinarum, a Parasitoid of the Coconut Leaf Beetle.Journal of chemical ecology · 2025Article
- Article
- Molecular Characterization of Chemosensory Protein (CSP) Genes and the Involvement ofInternational journal of molecular sciences · 2024Article
- Identification, expression profiles, and binding properties of chemosensory protein 18 in Plutella xylostella (Lepidoptera: Plutellidae).Journal of insect science (Online) · 2024Article
- Article
- Evolution of five environmentally responsive gene families in a pine-feeding sawfly,Ecology and evolution · 2023Article
- Expression Pattern and Ligand Binding Characteristics Analysis of Chemosensory Protein SnitCSP2 fromInsects · 2023Article
- A female-biased odorant receptor tuned to the lepidopteran sex pheromone in parasitoid Microplitis mediator guiding habitat of host insects.Journal of advanced research · 2023Article
- Transcriptome analysis and molecular characterization of soluble chemical communication proteins in the parasitoid waspEcology and evolution · 2022Article
- Spatial Expression Analysis of Odorant Binding Proteins in Both Sexes of the Aphid ParasitoidFrontiers in physiology · 2022Article
- Insights Into Chemosensory Proteins From Non-Model Insects: Advances and Perspectives in the Context of Pest Management.Frontiers in physiology · 2022Review
- Identification and expression profile of odorant-binding proteins in the parasitic wasp Microplitis pallidipes using PacBio long-read sequencing.Parasite (Paris, France) · 2022Article
- Antennal transcriptome sequencing and identification of candidate chemoreceptor proteins from an invasive pest, the American palm weevil, Rhynchophorus palmarum.Scientific reports · 2021Article
- Expression Profiles and Functional Characterization of Chemosensory Protein 15 (HhalCSP15) in the Brown Marmorated Stink BugFrontiers in physiology · 2021Article
- Article
- Molecular characterization and expression of sensory neuron membrane proteins in the parasitoid Microplitis mediator (Hymenoptera: Braconidae).Insect science · 2020Article
- The whole body transcriptome ofPeerJ · 2020Article
- Genome of the Parasitoid Wasp Diachasma alloeum, an Emerging Model for Ecological Speciation and Transitions to Asexual Reproduction.Genome biology and evolution · 2019Article
- Identification and expression of candidate chemosensory receptors in the white-spotted flower chafer, Protaetia brevitarsis.Scientific reports · 2019Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Odorant binding proteins (OBPs) and chemosensory proteins (CSPs) play important roles in transporting semiochemicals through the sensillar lymph to olfactory receptors in insect antennae. In the present study, twenty OBPs and three CSPs were identified from the antennal transcriptome of Microplitis mediator. Ten OBPs (MmedOBP11-20) and two CSPs (MmedCSP2-3) were newly identified. The expression patterns of these new genes in olfactory and non-olfactory tissues were investigated by real-time quantitative PCR (qPCR) measurement. The results indicated that MmedOBP14, MmedOBP18, MmedCSP2 and MmedCSP3 were primarily expressed in antennae suggesting potential olfactory roles in M. mediator. However, other genes including MmedOBP11-13, 15-17, 19-20 appeared to be expressed at higher levels in body parts than in antennae. Focusing on the functional characterization of MmedCSP3, immunocytochemistry and fluorescent competitive binding assays were conducted indoors. It was found that MmedCSP3 was specifically located in the sensillum lymph of olfactory sensilla basiconca type 2. The recombinant MmedCSP3 could bind several types of host insects odors and plant volatiles. Interestingly, three sex pheromone components of Noctuidae insects, cis-11-hexadecenyl aldehyde (Z11-16: Ald), cis-11-hexadecanol (Z11-16: OH), and trans-11-tetradecenyl acetate (E11-14: Ac), showed high binding affinities (Ki = 17.24-18.77 μM). The MmedCSP3 may be involved in locating host insects. Our data provide a base for further investigating the physiological roles of OBPs and CSPs in M. mediator, and extend the function of MmedCSP3 in chemoreception of M. mediator.
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