Evidence map›Paper›PMID 28730206›Full record

ArticleSoft matter2017

Evaluation of dextran(ethylene glycol) hydrogel films for giant unilamellar lipid vesicle production and their application for the encapsulation of polymersomes.

Nestor Lopez Mora, Yue Gao, M Gertrude Gutierrez, Justin Peruzzi, Ivan Bakker, Ruud J R W Peters, Bianka Siewert, Sylvestre Bonnet, Roxanne E Kieltyka, Jan C M van Hest and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Soft matter, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Nestor Lopez MoraLeiden Institute of Chemistry, Leiden University, Supramolecular & Biomaterials Chemistry, Einsteinweg 55, 2333 CC, Leiden, The Netherlands. r.e.kieltyka@chem.leidenuniv.nl.ORCID http://orcid.org/0000-0001-9803-6885
Yue GaoLeiden Institute of Chemistry, Leiden University, Supramolecular & Biomaterials Chemistry, Einsteinweg 55, 2333 CC, Leiden, The Netherlands. r.e.kieltyka@chem.leidenuniv.nl.
M Gertrude GutierrezDepartments of Chemical Engineering & Materials Science, Biomedical Engineering, and Chemistry, University of Southern California, 925 Bloom Walk, 90089, Los Angeles, CA, USA.
Justin PeruzziDepartment of Chemical Engineering, University of Virginia, 102 Engineer's Way, 400741, Charlottesville, VA, USA.
Ivan BakkerLeiden Institute of Chemistry, Leiden University, Supramolecular & Biomaterials Chemistry, Einsteinweg 55, 2333 CC, Leiden, The Netherlands. r.e.kieltyka@chem.leidenuniv.nl.
Ruud J R W PetersRadboud University Nijmegen, Department of Organic Chemistry, Heyendaalseweg 135 6525 AJ, Nijmegen, The Netherlands.
Bianka SiewertLeiden Institute of Chemistry, Leiden University, Metals in Catalysis, Biomimetics & Inorganic Materials, Einsteinweg 55, 2333 CC, Leiden, The Netherlands.ORCID http://orcid.org/0000-0002-4910-1756
Sylvestre BonnetLeiden Institute of Chemistry, Leiden University, Metals in Catalysis, Biomimetics & Inorganic Materials, Einsteinweg 55, 2333 CC, Leiden, The Netherlands.ORCID http://orcid.org/0000-0002-5810-3657
Roxanne E KieltykaLeiden Institute of Chemistry, Leiden University, Supramolecular & Biomaterials Chemistry, Einsteinweg 55, 2333 CC, Leiden, The Netherlands. r.e.kieltyka@chem.leidenuniv.nl.ORCID http://orcid.org/0000-0001-9152-1810
Jan C M van HestRadboud University Nijmegen, Department of Organic Chemistry, Heyendaalseweg 135 6525 AJ, Nijmegen, The Netherlands.
Noah MalmstadtDepartments of Chemical Engineering & Materials Science, Biomedical Engineering, and Chemistry, University of Southern California, 925 Bloom Walk, 90089, Los Angeles, CA, USA.
Alexander KrosLeiden Institute of Chemistry, Leiden University, Supramolecular & Biomaterials Chemistry, Einsteinweg 55, 2333 CC, Leiden, The Netherlands. r.e.kieltyka@chem.leidenuniv.nl.ORCID http://orcid.org/0000-0002-3983-3048

Funding

Connecting plasma membrane function to lipid structure and organization with asymR01GM093279 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI MALMSTADT, NOAH · 2010 to 2014
$1.4M
NIGMS NIH HHS R01 GM093279
6 · The paper itself

Abstract

Giant Unilamellar Vesicles (GUVs) prepared from phospholipids are becoming popular membrane model systems for use in biophysical studies. The quality, size and yield of GUVs depend on the preparation method used to obtain them. In this study, hydrogels consisting of dextran polymers crosslinked by poly(ethylene glycol) (DexPEG) were used as hydrophilic frameworks for the preparation of vesicle suspensions under physiological ionic strength conditions. A comparative study was conducted using hydrogels with varied physicochemical properties to evaluate their performance for GUV production. The prepared GUVs were quantified by flow cytometry using the Coulter Principle to determine the yield and size distribution. We find that hydrogels of lower mechanical strength, increased swellability and decreased lipid interaction favour GUV production, while their resulting size is determined by the surface roughness of the hydrogel film. Moreover, we embedded polymersomes into the crosslinked hydrogel network, creating a DexPEG - polymersome hybrid film. The re-hydration of lipids on those hybrid substrates led to the production of GUVs and the efficient encapsulation of polymersomes in the lumen of GUVs.

Identifiers

PMID28730206
PMCPMC5586486

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.