ArticleNature communications2017
Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 122 papers.
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Who cites it
122 citing papers in PubMed.
- Gut virome dynamics: from commensal to critical player in health and disease.Nature reviews. Gastroenterology & hepatology · 2026Review
- Metagenomic Data Preprocessing and Quality Control.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A genomic view of Earth's biomes.Nature reviews. Genetics · 2026Review
- Illuminating the newly produced viruses within the virosphere with bioorthogonal noncanonical amino acid tagging and single-virus genomic sequencing technologies.ISME communications · 2026Article
- Hybridization breaks species barriers in long-term coevolution of a cyanobacterial population.eLife · 2025Article
- Limited consensus of marine viral diversity observed across techniques.Environmental microbiome · 2025Article
- Single-particle genomics uncovers abundant non-canonical marine viruses from nanolitre volumes.Nature microbiology · 2025Article
- Bacterial immune systems as causes and consequences of microbiome structure.PLoS biology · 2025Article
- Mining Microbial Dark Matter: Advanced Cultivation Techniques for Bioactive Compound Discovery.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Profiling the heterogeneity of microbial populations and communities at the single-cell level.mLife · 2025Review
- Article
- Genetic isolation and metabolic complexity of an Antarctic subglacial microbiome.Nature communications · 2025Article
- Identification of multiple genomic alterations and prediction of neoantigens from circulating tumor cells at the single-cell level.Nature communications · 2025Article
- Hybridization breaks species barriers in long-term coevolution of a cyanobacterial population.bioRxiv : the preprint server for biology · 2025Article
- Exploring Environmental Microfungal Diversity Through Serial Single Cell Screening.Molecular ecology resources · 2025Article
- Promiscuous and genome-wide recombination underlies the sequence-discrete species of the SAR11 lineage in the deep ocean.The ISME journal · 2025Article
- Laminarin stimulates single cell rates of sulfate reduction whereas oxygen inhibits transcriptomic activity in coastal marine sediment.The ISME journal · 2025Article
- CleanBar: a versatile demultiplexing tool for split-and-pool barcoding in single-cell omics.ISME communications · 2025Article
- A novel isothermal whole genome sequencing approach for Monkeypox Virus.Scientific reports · 2024Article
- Genomic profiling of Antarctic geothermal microbiomes using long-read, Hi-C, and single-cell techniques.Scientific data · 2024Article
62 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial single-cell genomics can be used to provide insights into the metabolic potential, interactions, and evolution of uncultured microorganisms. Here we present WGA-X, a method based on multiple displacement amplification of DNA that utilizes a thermostable mutant of the phi29 polymerase. WGA-X enhances genome recovery from individual microbial cells and viral particles while maintaining ease of use and scalability. The greatest improvements are observed when amplifying high G+C content templates, such as those belonging to the predominant bacteria in agricultural soils. By integrating WGA-X with calibrated index-cell sorting and high-throughput genomic sequencing, we are able to analyze genomic sequences and cell sizes of hundreds of individual, uncultured bacteria, archaea, protists, and viral particles, obtained directly from marine and soil samples, in a single experiment. This approach may find diverse applications in microbiology and in biomedical and forensic studies of humans and other multicellular organisms.Single-cell genomics can be used to study uncultured microorganisms. Here, Stepanauskas et al. present a method combining improved multiple displacement amplification and FACS, to obtain genomic sequences and cell size information from uncultivated microbial cells and viral particles in environmental samples.
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