ArticleScientific reports2017
Cryopreservation protocol for human biliary tree stem/progenitors, hepatic and pancreatic precursors.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Biobanked Liver Organoids: A Roadmap for Precision Hepatology.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Cell transplantation-based regenerative medicine in liver diseases.Stem cell reports · 2023Review
- A postnatal network of co-hepato/pancreatic stem/progenitors in the biliary trees of pigs and humans.NPJ Regenerative medicine · 2023Article
- Biomolecular Pathways of Cryoinjuries in Low-Temperature Storage for Mammalian Specimens.Bioengineering (Basel, Switzerland) · 2022Review
- Therapeutic effects of dexamethasone-loaded hyaluronan nanogels in the experimental cholestasis.Drug delivery and translational research · 2022Article
- Human biliary epithelial cells from discarded donor livers rescue bile duct structure and function in a mouse model of biliary disease.Cell stem cell · 2022Article
- Patch grafting, strategies for transplantation of organoids into solid organs such as liver.Biomaterials · 2021Article
- Metformin exerts anti-cancerogenic effects and reverses epithelial-to-mesenchymal transition trait in primary human intrahepatic cholangiocarcinoma cells.Scientific reports · 2021Article
- DCLK1, a Putative Stem Cell Marker in Human Cholangiocarcinoma.Hepatology (Baltimore, Md.) · 2021Article
- Distinct EpCAM-Positive Stem Cell Niches Are Engaged in Chronic and Neoplastic Liver Diseases.Frontiers in medicine · 2020Review
- Article
- Review
- Hair-follicle-associated pluripotent stem cells derived from cryopreserved intact human hair follicles sustain multilineage differentiation potential.Scientific reports · 2019Article
- The FXR agonist obeticholic acid inhibits the cancerogenic potential of human cholangiocarcinoma.PloS one · 2019Article
- Contribution of Resident Stem Cells to Liver and Biliary Tree Regeneration in Human Diseases.International journal of molecular sciences · 2018Review
- Article
Corrections and comments
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Authors and funding
17 authors.
Funding
Abstract
Human biliary tree stem/progenitor cells (hBTSCs) are being used for cell therapies of patients with liver cirrhosis. A cryopreservation method was established to optimize sourcing of hBTSCs for these clinical programs and that comprises serum-free Kubota's Medium (KM) supplemented with 10% dimethyl sulfoxide (DMSO), 15% human serum albumin (HSA) and 0.1% hyaluronans. Cryopreserved versus freshly isolated hBTSCs were similar in vitro with respect to self-replication, stemness traits, and multipotency. They were able to differentiate to functional hepatocytes,cholangiocytes or pancreatic islets, yielding similar levels of secretion of albumin or of glucose-inducible levels of insulin. Cryopreserved versus freshly isolated hBTSCs were equally able to engraft into immunocompromised mice yielding cells with human-specific gene expression and human albumin levels in murine serum that were higher for cryopreserved than for freshly isolated hBTSCs. The successful cryopreservation of hBTSCs facilitates establishment of hBTSCs cell banking offering logistical advantages for clinical programs for treatment of liver diseases.
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Registered trials
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