ArticleParasites & vectors2017
Zika virus transmission to mouse ear by mosquito bite: a laboratory model that replicates the natural transmission process.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Zika virus: An updated review of competent or naturally infected mosquitoes.PLoS neglected tropical diseases · 2017Pooled it
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- The influence of culture-dependent native microbiota in Zika virus infection in Aedes aegypti.Parasites & vectors · 2022Article
- Dengue Infection Susceptibility of FiveViruses · 2021Article
- Dengue and Zika virus infection patterns vary among Aedes aegypti field populations from Belo Horizonte, a Brazilian endemic city.PLoS neglected tropical diseases · 2021Article
- Characterizing and Quantifying Arbovirus Transmission byInsects · 2021Article
- Pathogenesis and Immune Response of Ebinur Lake Virus: A Newly IdentifiedFrontiers in microbiology · 2020Article
- Article
- A simplified method for blood feeding, oral infection, and saliva collection of the dengue vector mosquitoes.PloS one · 2020Article
- Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) Delays Egg Hatching and Larval Development of Progeny.Journal of medical entomology · 2019Article
- Coinfection with Zika Virus (ZIKV) and Dengue Virus Results in Preferential ZIKV Transmission by Vector Bite to Vertebrate Host.The Journal of infectious diseases · 2018Article
- Establishment of a mouse model for the complete mosquito-mediated transmission cycle of Zika virus.PLoS neglected tropical diseases · 2018Article
- Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection.PLoS neglected tropical diseases · 2018Article
- Zika virus infection modulates the bacterial diversity associated with Aedes aegypti as revealed by metagenomic analysis.PloS one · 2018Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundZika disease has transformed into a serious global health problem due to the rapid spread of the arbovirus and alarming severity including congenital complications, microcephaly and Guillain-Barré syndrome. Zika virus (ZIKV) is primarily transmitted to humans through the bite of an infective mosquito, with Aedes aegypti being the main vector.
methodsWe successfully developed a ZIKV experimental transmission model by single infectious Ae. aegypti bite to a laboratory mouse using circulating Brazilian strains of both arbovirus and vector. Mosquitoes were orally infected and single Ae. aegypti were allowed to feed on mouse ears 14 days post-infection. Additionally, salivary gland (SG) homogenates from infected mosquitoes were intrathoracically inoculated into naïve Ae. aegypti. Mosquito and mouse tissue samples were cultured in C6/36 cells and processed by quantitative real-time PCR.
resultsA total of 26 Ae. aegypti were allowed to feed individually on mouse ears. Of these, 17 mosquitoes fed, all to full engorgement. The transmission rate of ZIKV by bite from these engorged mosquitoes to mouse ears was 100%. The amount of virus inoculated into the ears by bites ranged from 2 × 10
conclusionsOur model illustrates successful transmission of ZIKV by an infectious mosquito bite to a live vertebrate host. This approach offers a comprehensive tool for evaluating the development of infection in and transmission from mosquitoes, and the vertebrate-ZIKV interaction and progression of infection following a natural transmission process.
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