ArticleAsian-Australasian journal of animal sciences2018
Isolation and characterization of a novel gossypol-degrading bacteria
Article in Asian-Australasian journal of animal sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- Probiotic Fermentation of Defatted Cottonseed Meal for Sustainable Foods and Non-Food Applications.Microorganisms · 2025Review
- Use of Cottonseed Meal in Feeding Yellow-Feathered Broilers: Effects on Performance Parameters, Digestibility and Meat Quality.Veterinary sciences · 2025Article
- Effects of increased rapeseed meal addition on production performance, health, rumen fermentation, and microbial community in dairy goats.Journal of animal science · 2025Article
- Whole-genome sequencing and assessment of a novel protein- and gossypol-degrading Bacillus subtilis strain isolated from intestinal digesta of Tibetan Pigs.BMC microbiology · 2024Article
- Effects of replacing soybean meal with enzymolysis-fermentation compound protein feed on growth performance, apparent digestibility of nutrients, carcass traits, and meat quality in growing-finishing pigs.Journal of animal science and biotechnology · 2024Article
- Research progress on cottonseed meal as a protein source in pig nutrition: An updated review.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Review
- Integrative transcriptomic and metabolomic analysis to elucidate the effect of gossypol on Enterobacter sp. GD5.PloS one · 2024Article
- Effects of various supplemental levels of multi-enzyme complex on amino acid profiles in egg yolk, antioxidant capacity, cecal microbial community and metabolites of laying hens.Frontiers in microbiology · 2024Article
- Evaluating Fermentation Quality, Aerobic Stability, and Rumen-Degradation (In Situ) Characteristics of Various Protein-Based Total Mixed Rations.Animals : an open access journal from MDPI · 2023Article
- Biodegradation of Gossypol byMicroorganisms · 2023Article
- Fermented cottonseed and rapeseed meals outperform soybean meal in improving performance, rumen fermentation, and bacterial composition in Hu sheep.Frontiers in microbiology · 2023Article
- Response of a new rumen-derivedFrontiers in microbiology · 2023Article
- Effective gossypol removal from cottonseed meal through optimized solid-state fermentation by Bacillus coagulans.Microbial cell factories · 2022Article
- Biodegradation of Free Gossypol byToxins · 2022Article
- Cottonseed Meal Protein Isolate as a New Source of Alternative Proteins: A Proteomics Perspective.International journal of molecular sciences · 2022Article
- Essential Oils and Melatonin as Functional Ingredients in Dogs.Animals : an open access journal from MDPI · 2022Review
- Effect of lactic acid bacteria and yeast supplementation on anti-nutritional factors and chemical composition of fermented total mixed ration containing cottonseed meal or rapeseed meal.Animal bioscience · 2022Article
- Gossypol detoxification in the rumen andAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Review
- Isolation and Identification of a RumenMicroorganisms · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe aim of the study was to isolate gossypol-degrading bacteria and to assess its potential for gossypol degradation.
methodsRumen liquid was collected from fistulated cows grazing the experimental pasture. Approximately 1 mL of the rumen liquid was spread onto basal medium plates containing 2 g/L gossypol as the only source of carbon and was then cultured at 39°C to isolate gossypol-degrading bacteria. The isolated colonies were cultured for 6 h and then their size and shape observed by microscope and scanning electron microscope. The 16S rRNA gene of isolated colonies was sequenced and aligned using National Center for Biotechnology Information-Basic Local Alignment Search Tool. The various fermentation conditions, initial pH, incubation temperature, inoculum level and fermentationperiod were analyzed in cottonseed meal (CSM). The crude protein (CP), total gossypol (TG), and free gossypol (FG) were determined in CSM after fermentation with isolated strain at 39°C for 72 h.
resultsScreening results showed that a single bacterial isolate, named Rumen Bacillus Subtilis (RBS), could use gossypol as a carbon source. The bacterium was identified by 16S rDNA sequencing as being 98% homologous to the sequence of
conclusionThe isolation of a gossypol-degrading bacterium from the cow rumen is of great importance for gossypol biodegradation and may be a valuable potential source for gossypol-degradation of CSM.
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