ArticlePLoS genetics2017
Use of a microfluidic platform to uncover basic features of energy and environmental stress responses in individual cells of Bacillus subtilis.
Article in PLoS genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Stressosome-independent but RsbT-dependent environmental stress sensing in Bacillus subtilis.Nature communications · 2025Article
- Methods for studying microbial acid stress responses: from molecules to populations.FEMS microbiology reviews · 2024Review
- Temporal σmSphere · 2024Article
- Direct comparison of spatial transcriptional heterogeneity across diverse Bacillus subtilis biofilm communities.Nature communications · 2023Article
- The σB alternative sigma factor circuit modulates noise to generate different types of pulsing dynamics.PLoS computational biology · 2023Article
- Prediction and validation of novel SigB regulon members in Bacillus subtilis and regulon structure comparison to Bacillales members.BMC microbiology · 2023Article
- Article
- Molecular insights into intra-complex signal transmission during stressosome activation.Communications biology · 2022Article
- Proceedings from the 3rd International Conference on Microbiome Engineering.Biotechnology progress · 2022Article
- Acid stress signals are integrated into the σB-dependent general stress response pathway via the stressosome in the food-borne pathogen Listeria monocytogenes.PLoS pathogens · 2022Article
- Microfluidics for long-term single-cell time-lapse microscopy: Advances and applications.Frontiers in bioengineering and biotechnology · 2022Review
- Tunable phenotypic variability through an autoregulatory alternative sigma factor circuit.Molecular systems biology · 2021Article
- Stochastic pulsing of gene expression enables the generation of spatial patterns in Bacillus subtilis biofilms.Nature communications · 2020Article
- Management of Osmoprotectant Uptake Hierarchy inFrontiers in microbiology · 2020Article
- The σFrontiers in microbiology · 2020Review
- Blue Light Sensing inFrontiers in microbiology · 2019Article
- Molecular Time Sharing through Dynamic Pulsing in Single Cells.Cell systems · 2018Article
- Single-cell Microfluidic Analysis of Bacillus subtilis.Journal of visualized experiments : JoVE · 2018Article
- Analysis of Factors Limiting Bacterial Growth in PDMS Mother Machine Devices.Frontiers in microbiology · 2018Article
- Noise in a phosphorelay drives stochastic entry into sporulation inThe EMBO journal · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Bacteria use a variety of stress-sensing systems to sense and respond to diverse stressors and to ensure their survival under adverse conditions. The gram-positive bacterium Bacillus subtilis responds to energy stress (ATP depletion) and to environmental stressors using two distinct stress-sensing pathways that converge on the alternative sigma factor σB to provoke a general stress response. Past efforts to study the σB stress response in bulk culture and on agarose pads were unable to visualize the responses of individual cells under tightly controlled conditions for extended periods of time. Here we use a microfluidics-based strategy to discern the basic features of σB activation in single cells in response to energy and environmental stress, both immediately upon stressor exposure and for tens of generations thereafter. Upon energy stress at various levels of stressor, cells exhibited fast, transient, and amplitude-modulated responses but not frequency modulation as previously reported. Upon environmental stress, which is mediated by the stressosome complex, wild-type cells primarily exhibited a transient and amplitude-modulated response. However, mutant cells producing only one of the four paralogous RsbR stressosome proteins showed striking and previously unseen differences. Whereas RsbRA-only cells mimicked the wild type, RsbRC-only cells displayed a slower but sustained overall response composed of repeated activation events in single cells.
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Registered trials
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