ArticleIntegrative biology : quantitative biosciences from nano to macro2017
Engineering micromyocardium to delineate cellular and extracellular regulation of myocardial tissue contractility.
Article in Integrative biology : quantitative biosciences from nano to macro, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Harnessing native blueprints for designing bioinks to bioprint functional cardiac tissue.iScience · 2025Review
- Contractile responses of engineered humanAPL bioengineering · 2024Article
- Biomimetic Cardiac Tissue Models for In Vitro Arrhythmia Studies.Biomimetics (Basel, Switzerland) · 2023Review
- Regulation of oxytocin-induced calcium transients and gene expression in engineered myometrial tissues by tissue architecture and matrix rigidity.Current research in physiology · 2023Article
- Tools, techniques, and future opportunities for characterizing the mechanobiology of uterine myometrium.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Review
- Article
- Mitochondrial architecture in cardiac myocytes depends on cell shape and matrix rigidity.Journal of molecular and cellular cardiology · 2021Article
- Engineering the Cellular Microenvironment of Post-infarct Myocardium on a Chip.Frontiers in cardiovascular medicine · 2021Review
- Neuromuscular disease modeling on a chip.Disease models & mechanisms · 2020Review
- Matrix-guided control of mitochondrial function in cardiac myocytes.Acta biomaterialia · 2019Article
- Engineering hiPSC cardiomyocyte in vitro model systems for functional and structural assessment.Progress in biophysics and molecular biology · 2019Review
- Phenotypic Variation Between Stromal Cells Differentially Impacts Engineered Cardiac Tissue Function.Tissue engineering. Part A · 2019Article
- Three-dimensional microengineered models of human cardiac diseases.Journal of biological engineering · 2019Review
- Engineering cardiac microphysiological systems to model pathological extracellular matrix remodeling.American journal of physiology. Heart and circulatory physiology · 2018Review
- Microenvironmental Modulation of Calcium Wave Propagation Velocity in Engineered Cardiac Tissues.Cellular and molecular bioengineering · 2018Article
- Traction force microscopy of engineered cardiac tissues.PloS one · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Cardiovascular diseases are a leading cause of death, in part due to limitations of existing models of the myocardium. Myocardium consists of aligned, contractile cardiac myocytes interspersed with fibroblasts that synthesize extracellular matrix (ECM). The cellular demographics and biochemical and mechanical properties of the ECM remodel in many different cardiac diseases. However, the impact of diverse cellular and extracellular remodeling on the contractile output of the myocardium are poorly understood. To address this, we micropatterned 13 kPa and 90 kPa polyacrylamide gels with aligned squares of fibronectin (FN) or laminin (LN). We seeded gels with two concentrations of primary neonatal rat ventricular myocytes, which naturally contain fibroblasts. Cells assembled into aligned "μMyocardia" with fibroblast : myocyte ratios dependent on initial seeding concentration. Using traction force microscopy (TFM), we found that the peak systolic longitudinal cross-sectional force was similar across conditions, but the peak systolic work was significantly lower on 90 kPa gels. This indicates that ECM elasticity dominates over ECM ligand and cell demographics in regulating contractile output. Because our platform provides independent control over cell-cell and cell-matrix interactions, it has many applications for cardiac disease modeling.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.