Evidence map›Paper›PMID 28725925›Full record

ArticleExperimental brain research2017

Characterisation of Peptide5 systemic administration for treating traumatic spinal cord injured rats.

Yilin Mao, Tara Nguyen, Ryan S Tonkin, Justin G Lees, Caitlyn Warren, Simon J O'Carroll, Louise F B Nicholson, Colin R Green, Gila Moalem-Taylor, Catherine A Gorrie

Abstract read
PubMed Publisher
In one paragraph

Article in Experimental brain research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. The Potential Role of Connexins in the Pathogenesis of Atherosclerosis.International journal of molecular sciences · 2023
    Review
  2. Article
  3. Mechanisms of Connexin Regulating Peptides.International journal of molecular sciences · 2021
    Review
  4. Review
  5. Article
  6. Synthesis and biological evaluation ofRSC medicinal chemistry · 2020
    Article
  7. Review
  8. Article
  9. Review
  10. Connexin Hemichannels in Astrocytes: Role in CNS Disorders.Frontiers in molecular neuroscience · 2019
    Review
  11. Therapeutic strategies targeting connexins.Nature reviews. Drug discovery · 2018
    Review
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yilin MaoNeural Injury Research Unit, School of Life Sciences, Faculty of Science, University of Technology Sydney, P.O. Box 123, Broadway, NSW, 2007, Australia.
Tara NguyenNeural Injury Research Unit, School of Life Sciences, Faculty of Science, University of Technology Sydney, P.O. Box 123, Broadway, NSW, 2007, Australia.
Ryan S TonkinNeuropathic Pain Research Group, Translational Neuroscience Facility, School of Medical Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
Justin G LeesNeuropathic Pain Research Group, Translational Neuroscience Facility, School of Medical Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
Caitlyn WarrenNeural Injury Research Unit, School of Life Sciences, Faculty of Science, University of Technology Sydney, P.O. Box 123, Broadway, NSW, 2007, Australia.
Simon J O'CarrollDepartment of Anatomy and Medical Imaging and The Centre for Brain Research, Faculty of Medical and Health Sciences, University of Auckland, Auckland, 1142, New Zealand.
Louise F B NicholsonDepartment of Anatomy and Medical Imaging and The Centre for Brain Research, Faculty of Medical and Health Sciences, University of Auckland, Auckland, 1142, New Zealand.
Colin R GreenDepartment of Ophthalmology and The New Zealand National Eye Centre, Faculty of Medical and Health Sciences, University of Auckland, Auckland, 1142, New Zealand.
Gila Moalem-TaylorNeuropathic Pain Research Group, Translational Neuroscience Facility, School of Medical Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
Catherine A GorrieNeural Injury Research Unit, School of Life Sciences, Faculty of Science, University of Technology Sydney, P.O. Box 123, Broadway, NSW, 2007, Australia. Catherine.Gorrie@uts.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Systemic administration of a Connexin43 mimetic peptide, Peptide5, has been shown to reduce secondary tissue damage and improve functional recovery after spinal cord injury (SCI). This study investigated safety measures and potential off-target effects of Peptide5 systemic administration. Rats were subjected to a mild contusion SCI using the New York University impactor. One cohort was injected intraperitoneally with a single dose of fluorescently labelled Peptide5 and euthanised at 2 or 4 h post-injury for peptide distribution analysis. A second cohort received intraperitoneal injections of Peptide5 or a scrambled peptide and was culled at 8 or 24 h post-injury for the analysis of connexin proteins and systemic cytokine profile. We found that Peptide5 did not cross the blood-spinal cord barrier in control animals, but reached the lesion area in the spinal cord-injured animals without entering non-injured tissue. There was no evidence that the systemic administration of Peptide5 modulates Connexin43 protein expression or hemichannel closure in the heart and lung tissue of SCI animals. The expression levels of other major connexin proteins including Connexin30 in astrocytes, Connexin36 in neurons and Connexin47 in oligodendrocytes were also unaltered by systemic delivery of Peptide5 in either the injured or non-injured spinal cords. In addition, systemic delivery of Peptide5 had no significant effect on the plasma levels of cytokines, chemokines or growth factors. These data indicate that the systemic delivery of Peptide5 is unlikely to cause any off-target or adverse effects and may thus be a safe treatment option for traumatic SCI.

Indexed as

AnimalsBiomimetic MaterialsConnexin 43Disease Models, AnimalFemaleRatsRats, Sprague-DawleySpinal Cord InjuriesConnexin 43Connexin43 mimetic peptidesConnexinsSafety profileSpinal cord injurySystemic administrationTissue distribution

Identifiers

PMID28725925

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.