ArticleNPJ microgravity2015
Effects of angular frequency during clinorotation on mesenchymal stem cell morphology and migration.
Article in NPJ microgravity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Impact of Microgravity on Cytoskeletal Dynamics, Protein Transport, and Signaling Networks: Potential Therapeutic Opportunities for Skin Health.International journal of molecular sciences · 2026Review
- Dielectric double shell characterization of yeast cells exposed to simulated microgravity.NPJ microgravity · 2026Article
- Characterization of the random positioning machine as a microgravity simulator for biological applications.PloS one · 2026Article
- Surface tension enables induced pluripotent stem cell culture in commercially available hardware during spaceflight.NPJ microgravity · 2024Article
- Fluid and Bubble Flow Detach Adherent Cancer Cells to Form Spheroids on a Random Positioning Machine.Cells · 2023Article
- EuniceScope: Low-Cost Imaging Platform for Studying Microgravity Cell Biology.IEEE open journal of engineering in medicine and biology · 2023Article
- Lab-on-a-Chip Technologies for Microgravity Simulation and Space Applications.Micromachines · 2022Review
- 3D cell culture using a clinostat reproduces microgravity-induced skin changes.NPJ microgravity · 2021Article
- Gravity sensing in plant and animal cells.NPJ microgravity · 2021Review
- Testing Lab-on-a-Chip Technology for Culturing Human Melanoma Cells under Simulated Microgravity.Cancers · 2021Article
- Feasibility, potency, and safety of growing human mesenchymal stem cells in space for clinical application.NPJ microgravity · 2020Article
- Response of extreme haloarchaeon Haloarcula argentinensis RR10 to simulated microgravity in clinorotation.3 Biotech · 2017Article
- Impact of Simulated Microgravity on Cytoskeleton and Viscoelastic Properties of Endothelial Cell.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo determine the short-term effects of simulated microgravity on mesenchymal stem cell behaviors-as a function of clinorotation speed-using time-lapse microscopy.
backgroundGround-based microgravity simulation can reproduce the apparent effects of weightlessness in spaceflight using clinostats that continuously reorient the gravity vector on a specimen, creating a time-averaged nullification of gravity. In this work, we investigated the effects of clinorotation speed on the morphology, cytoarchitecture, and migration behavior of human mesenchymal stem cells (hMSCs).
methodsWe compared cell responses at clinorotation speeds of 0, 30, 60, and 75 rpm over 8 h in a recently developed lab-on-chip-based clinostat system. Time-lapse light microscopy was used to visualize changes in cell morphology during and after cessation of clinorotation. Cytoarchitecture was assessed by actin and vinculin staining, and chemotaxis was examined using time-lapse light microscopy of cells in NGF (100 ng/ml) gradients.
resultsAmong clinorotated groups, cell area distributions indicated a greater inhibition of cell spreading with higher angular frequency (
conclusionsThese results indicate that hMSCs respond to clinorotation by adopting more rounded, less-spread morphologies. The angular frequency-dependence suggests that a cell's ability to sense the changing gravity vector is governed by the rate of perturbation. For migration studies, cells cultured in clinorotated chemotaxis chambers were generally less motile and exhibited retraction instead of migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.