ArticleBiotechnology for biofuels2017
Two bifunctional enzymes from the marine protist
Article in Biotechnology for biofuels, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 33 citations in OpenAlex.
- Identification and Analysis of Cuticular Wax Biosynthesis Related Genes inInternational journal of molecular sciences · 2025Article
- Characterization of Auxenochlorella protothecoides acyltransferases and potential of their protein interactions to promote the enrichment of oleic acid.Biotechnology for biofuels and bioproducts · 2023Article
- Microbiome differences in sugarcane and metabolically engineered oilcane accessions and their implications for bioenergy production.Biotechnology for biofuels and bioproducts · 2023Article
- Engineering caveolin-mediated endocytosis inSynthetic and systems biotechnology · 2022Article
- The production of wax esters in transgenic plants: towards a sustainable source of bio-lubricants.Journal of experimental botany · 2022Review
- Genome-wide analysis of the WSD family in sunflower and functional identification ofFrontiers in plant science · 2022Article
- Overexpression of Two New Acyl-CoA:Diacylglycerol Acyltransferase 2-Like Acyl-CoA:Sterol Acyltransferases Enhanced Squalene Accumulation inFrontiers in microbiology · 2022Article
- The Studies in Constructing Yeast Cell Factories for the Production of Fatty Acid Alkyl Esters.Frontiers in bioengineering and biotechnology · 2021Review
- Structural and Biochemical Studies of a Biocatalyst for the Enzymatic Production of Wax Esters.ACS catalysis · 2018Article
- Characterization and engineering of a dual-function diacylglycerol acyltransferase in the oleaginous marine diatomBiotechnology for biofuels · 2018Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTriacylglycerols (TAGs) and wax esters (WEs) are important neutral lipids which serve as energy reservoir in some plants and microorganisms. In recent years, these biologically produced neutral lipids have been regarded as potential alternative energy sources for biofuel production because of the increased interest on developing renewable and environmentally benign alternatives for fossil fuels. In bacteria, the final step in TAG and WE biosynthetic pathway is catalyzed by wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase (WS/DGAT). This bifunctional WS/DGAT enzyme is also a key enzyme in biotechnological production of liquid WE via engineering of plants and microorganisms. To date, knowledge about this class of biologically and biotechnologically important enzymes is mainly from biochemical characterization of WS/DGATs from Arabidopsis, jojoba and some bacteria that can synthesize both TAGs and WEs intracellularly, whereas little is known about WS/DGATs from eukaryotic microorganisms.
resultsHere, we report the identification and characterization of two bifunctional WS/DGAT enzymes (designated TrWSD4 and TrWSD5) from the marine protist
conclusionsThis study demonstrates both in vitro WS and DGAT activity of two
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