ArticleScientific reports2017
Detection and quantitation of copy number variation in the voltage-gated sodium channel gene of the mosquito Culex quinquefasciatus.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Trends in insecticide resistance in Anopheles mosquitoes (Diptera: Culicidae) in Ghana: a systematic review.Journal of medical entomology · 2025Pooled it
- Does use of domestic insecticides undermine public health control strategies?Lancet regional health. Americas · 2025Review
- Omics Approaches in Understanding Insecticide Resistance in Mosquito Vectors.International journal of molecular sciences · 2025Review
- The dynamics of deltamethrin resistance evolution in Aedes albopictus has an impact on fitness and dengue virus type-2 vectorial capacity.BMC biology · 2023Article
- Widespread occurrence of copy number variants and fixation of pyrethroid target site resistance inCurrent research in parasitology & vector-borne diseases · 2023Article
- Detection of sex in adults and larvae of Leptinotarsa decemlineata on principle of copy number variation.Scientific reports · 2022Article
- Detection of the Nav channel kdr-like mutation and modeling of factors affecting survivorship of Culex quinquefasciatus mosquitoes from six areas of Harris County (Houston), Texas, after permethrin field-cage tests.PLoS neglected tropical diseases · 2020Article
- Population dynamics, pathogen detection and insecticide resistance of mosquito and sand fly in refugee camps, Greece.Infectious diseases of poverty · 2020Article
- Effects of Propoxur Exposure on Insecticidal Susceptibility and Developmental Traits inInsects · 2019Article
- Transcriptomic analysis of insecticide resistance in the lymphatic filariasis vector Culex quinquefasciatus.Scientific reports · 2019Article
- Whole-genome sequencing reveals high complexity of copy number variation at insecticide resistance loci in malaria mosquitoes.Genome research · 2019Article
- Development and application of a tri-allelic PCR assay for screening Vgsc-L1014F kdr mutations associated with pyrethroid and organochlorine resistance in the mosquito Culex quinquefasciatus.Parasites & vectors · 2019Article
- Article
- Copy number variation (CNV) and insecticide resistance in mosquitoes: evolving knowledge or an evolving problem?Current opinion in insect science · 2018Review
- Local selection in the presence of high levels of gene flow: Evidence of heterogeneous insecticide selection pressure across Ugandan Culex quinquefasciatus populations.PLoS neglected tropical diseases · 2017Article
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7 authors.
Funding
Abstract
Insecticide resistance is typically associated with alterations to the insecticidal target-site or with gene expression variation at loci involved in insecticide detoxification. In some species copy number variation (CNV) of target site loci (e.g. the Ace-1 target site of carbamate insecticides) or detoxification genes has been implicated in the resistance phenotype. We show that field-collected Ugandan Culex quinquefasciatus display CNV for the voltage-gated sodium channel gene (Vgsc), target-site of pyrethroid and organochlorine insecticides. In order to develop field-applicable diagnostics for Vgsc CN, and as a prelude to investigating the possible association of CN with insecticide resistance, three assays were compared for their accuracy in CN estimation in this species. The gold standard method is droplet digital PCR (ddPCR), however, the hardware is prohibitively expensive for widespread utility. Here, ddPCR was compared to quantitative PCR (qPCR) and pyrosequencing. Across all platforms, CNV was detected in ≈10% of mosquitoes, corresponding to three or four copies (per diploid genome). ddPCR and qPCR-Std-curve yielded similar predictions for Vgsc CN, indicating that the qPCR protocol developed here can be applied as a diagnostic assay, facilitating monitoring of Vgsc CN in wild populations and the elucidation of association between the Vgsc CN and insecticide resistance.
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