ArticleScientific reports2017
Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Article
- A method of comprehensive sequencing analysis of the small RNA fragmentome (RiboMarker).RNA (New York, N.Y.) · 2026Article
- Practical RNA-Seq with Spike-Ins: A Bench-to-Bioinformatics Guide.Methods in molecular biology (Clifton, N.J.) · 2026Article
- An ancient and essential miRNA family controls cellular interaction pathways inScience advances · 2025Article
- Mime-seq 2.0: a method to sequence microRNAs from specific mouse cell types.The EMBO journal · 2024Article
- The plasma miRNome and venous thromboembolism in high-grade glioma: miRNA Sequencing of a nested case-control cohort.Journal of cellular and molecular medicine · 2024Article
- Transcriptome-wide profiling of acute stress induced changes in ribosome occupancy level using external standards.PloS one · 2023Article
- Distinct regulatory pathways contribute to dynamic CHH methylation patterns in transposable elements throughoutFrontiers in plant science · 2023Article
- A MicroRNA Next-Generation-Sequencing Discovery Assay (miND) for Genome-Scale Analysis and Absolute Quantitation of Circulating MicroRNA Biomarkers.International journal of molecular sciences · 2022Article
- Article
- Cardiovascular RNA markers and artificial intelligence may improve COVID-19 outcome: a position paper from the EU-CardioRNA COST Action CA17129.Cardiovascular research · 2021Article
- Chromatin regulates expression of small RNAs to help maintain transposon methylome homeostasis in Arabidopsis.Genome biology · 2020Article
- Custom selected reference genes outperform pre-defined reference genes in transcriptomic analysis.BMC genomics · 2020Article
- MicroRNA Dynamics and Functions During Arabidopsis Embryogenesis.The Plant cell · 2019Article
- Bias-minimized quantification of microRNA reveals widespread alternative processing and 3' end modification.Nucleic acids research · 2019Article
- The embryonic transcriptome of Arabidopsis thaliana.Plant reproduction · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
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Abstract
Normalization of high-throughput small RNA sequencing (sRNA-Seq) data is required to compare sRNA levels across different samples. Commonly used relative normalization approaches can cause erroneous conclusions due to fluctuating small RNA populations between tissues. We developed a set of sRNA spike-in oligonucleotides (sRNA spike-ins) that enable absolute normalization of sRNA-Seq data across independent experiments, as well as the genome-wide estimation of sRNA:mRNA stoichiometries when used together with mRNA spike-in oligonucleotides.
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Registered trials
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