ArticleScientific reports2017
Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 44 citations in OpenAlex.
- Intracellular Relaxometry, Challenges, and Future Directions.ACS central science · 2022Review
- Article
- pH Sensitive Dextran Coated Fluorescent Nanodiamonds as a Biomarker for HeLa Cells Endocytic Pathway and Increased Cellular Uptake.Nanomaterials (Basel, Switzerland) · 2021Article
- Quantum monitoring of cellular metabolic activities in single mitochondria.Science advances · 2021Article
- Targeting Nanodiamonds to the Nucleus in Yeast Cells.Nanomaterials (Basel, Switzerland) · 2020Article
- Simultaneous label-free live imaging of cell nucleus and luminescent nanodiamonds.Scientific reports · 2020Article
- The Fate of Lipid-Coated and Uncoated Fluorescent Nanodiamonds during Cell Division in Yeast.Nanomaterials (Basel, Switzerland) · 2020Article
- Evaluation of the Oxidative Stress Response of Aging Yeast Cells in Response to Internalization of Fluorescent Nanodiamond Biosensors.Nanomaterials (Basel, Switzerland) · 2020Article
- Bioengineering tools for the production of pharmaceuticals: current perspective and future outlook.Bioengineered · 2019Review
- A Perspective on Fluorescent Nanodiamond Bioimaging.Small (Weinheim an der Bergstrasse, Germany) · 2019Review
- The Response of HeLa Cells to Fluorescent NanoDiamond Uptake.Sensors (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescent nanodiamonds (FNDs) are promising nanoprobes, owing to their stable and magnetosensitive fluorescence. Therefore they can probe properties as magnetic resonances, pressure, temperature or strain. The unprecedented sensitivity of diamond defects can detect the faint magnetic resonance of a single electron or even a few nuclear spins. However, these sensitivities are only achieved if the diamond probe is close to the molecules that need to be detected. In order to utilize its full potential for biological applications, the diamond particle has to enter the cell. Some model systems, like HeLa cells, readily ingest particles. However, most cells do not show this behavior. In this article we show for the first time generally applicable methods, which are able to transport fluorescent nanodiamonds into cells with a thick cell wall. Yeast cells, in particular Saccharomyces cerevisiae, are a favored model organism to study intracellular processes including aging on a cellular level. In order to introduce FNDs in these cells, we evaluated electrical transformation and conditions of chemical permeabilization for uptake efficiency and viability. 5% DMSO (dimethyl sulfoxide) in combination with optimized chemical transformation mix leads to high uptake efficiency in combination with low impact on cell biology. We have evaluated all steps in the procedure.
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