ArticleAngewandte Chemie (International ed. in English)2017
RadH: A Versatile Halogenase for Integration into Synthetic Pathways.
Article in Angewandte Chemie (International ed. in English), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 62 citations in OpenAlex.
- Precision Molecular Editing: Predicting Substrate Scope and Regiochemistry for CHEESY1, a Flavin Dependent Halogenase.ACS catalysis · 2025Article
- Regioselective C─H Functionalization by the Combination of Enzymatic and Chemocatalytic Reactions in Water.Angewandte Chemie (International ed. in English) · 2025Article
- Aerobic oxidative bromination and iodination enabled by alloxan and ascorbic acid to mimic flavin-dependent halogenases.Nature communications · 2025Article
- Combinatorial biosynthesis for the engineering of novel fungal natural products.Communications chemistry · 2024Review
- A modular and synthetic biosynthesis platform for de novo production of diverse halogenated tryptophan-derived molecules.Nature communications · 2024Article
- Characterization of Dichloroisoeverninic Acid Biosynthesis and Chemoenzymatic Synthesis of New Orthosomycins.ACS chemical biology · 2024Article
- Site-selective chlorination of pyrrolic heterocycles by flavin dependent enzyme PrnC.Communications chemistry · 2024Article
- The Lichen Flavin-Dependent Halogenase, DnHal: Identification, Heterologous Expression and Functional Characterization.Applied biochemistry and biotechnology · 2023Article
- Characterization of the depsidone gene cluster reveals etherification, decarboxylation and multiple halogenations as tailoring steps in depsidone assembly.Acta pharmaceutica Sinica. B · 2023Article
- Further Characterization of Fungal Halogenase RadH and Its Homologs.Biomolecules · 2023Article
- Indole and azaindole halogenation catalyzed by the RebH enzyme variant 3-LSR utilizing co-purifiedFrontiers in bioengineering and biotechnology · 2022Article
- A Promiscuous Halogenase for the Derivatization of Flavonoids.Molecules (Basel, Switzerland) · 2021Article
- Engineered RebH Halogenase Variants Demonstrating a Specificity Switch from Tryptophan towards Novel Indole Compounds.Chembiochem : a European journal of chemical biology · 2021Article
- Halogenases: a palette of emerging opportunities for synthetic biology-synthetic chemistry and C-H functionalisation.Chemical Society reviews · 2021Review
- Synthetic C6-Functionalized Aminoflavin Catalysts Enable Aerobic Bromination of Oxidation-Prone Substrates.Angewandte Chemie (International ed. in English) · 2020Article
- Engineering Plant Synthetic Pathways for the Biosynthesis of Novel Antifungals.ACS central science · 2020Article
- Structure of apo flavin-dependent halogenase Xcc4156 hints at a reason for cofactor-soaking difficulties.Acta crystallographica. Section D, Structural biology · 2020Article
- Site-Selective C-H Halogenation Using Flavin-Dependent Halogenases Identified via Family-Wide Activity Profiling.ACS central science · 2019Article
- Halogenating Enzymes for Active Agent Synthesis: First Steps Are Done and Many Have to Follow.Molecules (Basel, Switzerland) · 2019Review
- Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in Escherichia coli.PLoS biology · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Flavin-dependent halogenases are useful enzymes for providing halogenated molecules with improved biological activity, or intermediates for synthetic derivatization. We demonstrate how the fungal halogenase RadH can be used to regioselectively halogenate a range of bioactive aromatic scaffolds. Site-directed mutagenesis of RadH was used to identify catalytic residues and provide insight into the mechanism of fungal halogenases. A high-throughput fluorescence screen was also developed, which enabled a RadH mutant to be evolved with improved properties. Finally we demonstrate how biosynthetic genes from fungi, bacteria, and plants can be combined to encode a new pathway to generate a novel chlorinated coumarin "non-natural" product in E. coli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.