ArticleGenesis (New York, N.Y. : 2000)2017
Male germline recombination of a conditional allele by the widely used Dermo1-cre (Twist2-cre) transgene.
Article in Genesis (New York, N.Y. : 2000), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- Spatiotemporal Regulation and Lineage Specification in Embryonic Endochondral Ossification.International journal of molecular sciences · 2026Review
- TWIST1, a gene associated with Saethre-Chotzen syndrome, regulates extraocular muscle organization in mouse.Developmental biology · 2022Article
- Expression of Cre recombinase in chondrocytes causes abnormal craniofacial and skeletal development.Transgenic research · 2022Article
- The Wnt1-Cre2 transgene is active in the male germline.Genesis (New York, N.Y. : 2000) · 2022Article
- Phenotypic Diversity Caused by Differential Expression ofAmerican journal of respiratory cell and molecular biology · 2020Article
- Ectopic germline recombination activity of the widely used Foxp3-YFP-Cre mouse: a case report.Immunology · 2020Article
- Off-Target Deletion of ConditionalCells · 2019Article
- Mid-facial developmental defects caused by the widely used LacZ reporter gene when expressed in neural crest-derived cells.Transgenic research · 2018Article
- Cre-Recombinase Dependent Germline Deletion of a Conditional Allele in the Rgs9cre Mouse Line.Frontiers in neural circuits · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Conditional gene knockout using the Cre/loxP system is instrumental in advancing our understanding of the function of genes in a wide range of disciplines. It is becoming increasingly apparent in the literature that recombination mediated by some Cre transgenes can occur in unexpected tissues. Dermo1-Cre (Twist2-Cre) has been widely used to target skeletal lineage cells as well as other mesoderm-derived cells. Here we report that Dermo1-Cre exhibits spontaneous male germline recombination activity leading to a Cre-mediated recombination of a floxed Ptk2 (Protein tyrosine kinase 2, also known as Fak [Focal adhesion kinase]) allele but not a floxed Rb1cc1 (RB1 inducible coiled-coil 1, also known as Fip200 [FAK-family Interacting Protein of 200 kDa]) allele at high frequency. This ectopic germline activity of Dermo1-Cre occurred in all or none manner in a given litter. We demonstrated that the occurrence of germline recombination activity of Dermo1-Cre transgene can be avoided by using female mice as parental Dermo1-Cre carriers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.