Evidence map›Paper›PMID 28721511›Full record

ArticleTropical animal health and production2017

Molecular and phylogenetic characterization of bovine coronavirus virus isolated from dairy cattle in Central Region, Thailand.

Kanokwan Singasa, Taweesak Songserm, Preeda Lertwatcharasarakul, Pipat Arunvipas

Open access · bronzeAbstract read
In one paragraph

Article in Tropical animal health and production, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Review
  6. Review a brief history of coronaviruses in Thailand.Journal of virological methods · 2021
    Review
  7. Review
  8. Coronaviruses in cattle.Tropical animal health and production · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Kanokwan SingasaDepartment of Large Animal and Wildlife Clinical Science, Faculty of Veterinary Medicine, Kasetsart University, Kampangsaen Campus, 1 Malaiman Road, Kamphaeng Saen, Kamphaeng Saen District, Nakhon Pathom, 73140, Thailand.
Taweesak SongsermDepartment of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Kampangsaen Campus, Nakhon Pathom, Thailand.
Preeda LertwatcharasarakulDepartment of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Kampangsaen Campus, Nakhon Pathom, Thailand.
Pipat ArunvipasDepartment of Large Animal and Wildlife Clinical Science, Faculty of Veterinary Medicine, Kasetsart University, Kampangsaen Campus, 1 Malaiman Road, Kamphaeng Saen, Kamphaeng Saen District, Nakhon Pathom, 73140, Thailand. fvetpia@ku.ac.th.ORCID http://orcid.org/0000-0003-4178-5273
Kasetsart University · TH

Funding

Graduate School, Kasetsart University KU 72 year Anniversary Graduate Scholarship
6 · The paper itself

Abstract

Bovine coronavirus (BCoV) is involved mainly in enteric infections in cattle. This study reports the first molecular detection of BCoV in a diarrhea outbreak in dairy cows in the Central Region, Thailand. BCoV was molecularly detected from bloody diarrheic cattle feces by using nested PCR. Agarose gel electrophoresis of three diarrheic fecal samples yielded from the 25 samples desired amplicons that were 488 base pairs and sequencing substantiated that have BCoV. The sequence alignment indicated that nucleotide and amino acid sequences, the three TWD isolated in Thailand, were more quite homologous to each other (amino acid at position 39 of TWD1, TWD3 was proline, but TWD2 was serine) and closely related to OK-0514-3strain (virulent respiratory strain; RBCoV).The amino acid sequencing identities among TWD1, TWD2,TWD3, and OK-0514-3 strain were 96.0 to 96.6%, those at which T3I, H65N, D87G, H127Y, andQ136R were changed. In addition, the phylogenetic tree of the hypervariable region S1subunit spike glycoprotein BCoV gene was composed of three major clades by using the 54 sequences generated and showed that the evolutionally distance, TWD1, TWD2, and TWD3 were the isolated group together and most similar to OK-0514-3 strain (98.2 to 98.5% similarity). Further study will develop ELISA assay for serologic detection of winter dysentery disease.

Indexed as

AnimalsCattleCattle DiseasesCoronavirus, BovineCoronavirus InfectionsDiarrheaFecesFemalePhylogenySequence Analysis, RNAThailandBovine coronavirusDairy cattleNested PCRWinter dysentery disease

Identifiers

PMID28721511
PMCPMC7089240
OpenAlexW2739456504

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.