ArticleTranslational vision science & technology2017
Retinal Pigment Epithelial Cells are a Potential Reservoir for Ebola Virus in the Human Eye.
Article in Translational vision science & technology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 51 citations in OpenAlex.
- Putative mechanisms of ocular inflammation in syphilis.Journal of ophthalmic inflammation and infection · 2026Review
- Host-virus miRNA crosstalk in ebola virus disease.Folia microbiologica · 2026Review
- Molecular responses of human retinal pigment epithelial cells to ebolavirus VP24.Molecular vision · 2026Article
- Dengue-Related Ocular Complications: Spectrum, Diagnosis, and Management.Pathogens (Basel, Switzerland) · 2025Review
- Identification and Potential Roles of Human MicroRNAs in Ebola Virus Infection and Disease Pathogenesis.Genes · 2024Article
- Cutaneous granulomas associated with rubella virus: A clinical review.Journal of the American Academy of Dermatology · 2024Review
- Article
- Ebola Virus Tropism in Ex Vivo Cynomolgus Macaque Ocular Tissues.The Journal of infectious diseases · 2023Article
- Characterization of Ebola Virus Mucosal Challenge Routes in Cynomolgus Macaques.Journal of virology · 2023Article
- Persistent intraocular Ebola virus RNA is associated with severe uveitis in a convalescent rhesus monkey.Communications biology · 2022Article
- Systemic Candida albicans Infection in Mice Causes Endogenous Endophthalmitis via Breaching the Outer Blood-Retinal Barrier.Microbiology spectrum · 2022Article
- Infection of Human Retinal Pigment Epithelial Cells with Dengue Virus Strains Isolated during Outbreaks in Singapore.Microorganisms · 2022Article
- SARS-CoV-2 and its beta variant of concern infect human conjunctival epithelial cells and induce differential antiviral innate immune response.The ocular surface · 2022Article
- Immune Privilege Furnishes a Niche for Latent Infection.Frontiers in ophthalmology · 2022Review
- Dose-Dependent Response to Infection with Ebola Virus in the Ferret Model and Evidence of Viral Evolution in the Eye.Journal of virology · 2021Article
- Zika Virus Infection of Human Iris Pigment Epithelial Cells.Frontiers in immunology · 2021Article
- The state of latency in microbial pathogenesis.The Journal of clinical investigation · 2020Review
- Approach to Cataract Surgery in an Ebola Virus Disease Survivor with Prior Ocular Viral Persistence.Emerging infectious diseases · 2020Article
- Pathogenesis of Uveitis in Ebola Virus Disease Survivors: Evolving Understanding from Outbreaks to Animal Models.Microorganisms · 2020Review
- Insights into the Recent 2019 Novel Coronavirus (SARS-CoV-2) in Light of Past Human Coronavirus Outbreaks.Pathogens (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeSuccess of Ebola virus (EBOV) as a human pathogen relates at the molecular level primarily to blockade the host cell type I interferon (IFN) antiviral response. Most individuals who survive Ebola virus disease (EVD) develop a chronic disease syndrome: approximately one-quarter of survivors suffer from uveitis, which has been associated with presence of EBOV within the eye. Clinical observations of post-Ebola uveitis indicate involvement of retinal pigment epithelial cells.
methodsWe inoculated ARPE-19 human retinal pigment epithelial cells with EBOV, and followed course of infection by immunocytochemistry and measurement of titer in culture supernatant. To interrogate transcriptional responses of infected cells, we combined RNA sequencing with in silico pathway, gene ontology, transcription factor binding site, and network analyses. We measured infection-induced changes of selected transcripts by reverse transcription-quantitative polymerase chain reaction.
resultsHuman retinal pigment epithelial cells were permissive to infection with EBOV, and supported viral replication and release of virus in high titer. Unexpectedly, 28% of 560 upregulated transcripts in EBOV-infected cells were type I IFN responsive, indicating a robust type I IFN response. Following EBOV infection, cells continued to express multiple immunomodulatory molecules linked to ocular immune privilege.
conclusionsHuman retinal pigment epithelial cells may serve as an intraocular reservoir for EBOV, and the molecular response of infected cells may contribute to the persistence of live EBOV within the human eye. TRANSLATIONAL RELEVANCE: This bedside-to-bench research links ophthalmic findings in survivors of EVD who suffer from uveitis with interactions between retinal pigment epithelial cells and EBOV.
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