ArticleScientific reports2017
Melatonin promotes osteoblast differentiation by regulating Osterix protein stability and expression.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 79 citations in OpenAlex.
- The Circadian-Melatonin Axis in Bone Remodeling: Receptor-Dependent Signaling, Receptor-Independent Actions, and Translational Constraints.Biomolecules · 2026Review
- The circadian-melatonin axis in breast cancer metastasis: molecular mechanisms, organ-specific metastatic niches, and therapeutic opportunities.Frontiers in endocrinology · 2026Review
- Effect of melatonin on the osteogenic differentiation of periodontal ligament stem cells: in vitro study.BMC oral health · 2025Article
- Melatonin: A Potential Therapy for Osteoporosis With Insights Into Molecular Mechanisms.Journal of pineal research · 2025Review
- Aberrant Tryptophan Metabolism Manipulates Osteochondral Homeostasis.Research (Washington, D.C.) · 2025Review
- Epigenetic Mechanisms in Osteoporosis: Exploring the Power of mJournal of cellular and molecular medicine · 2025Review
- Genetics and pathogenesis of scoliosis.North American Spine Society journal · 2024Article
- TRIM21 silencing inhibits the apoptosis and expedites the osteogenic differentiation of dexamethasone‑induced MC3T3‑E1 cells by activating the Keap1/Nrf2 pathway.Experimental and therapeutic medicine · 2024Article
- Melatonin as a multifunctional modulator: emerging insights into its role in health, reproductive efficiency, and productive performance in livestock.Frontiers in physiology · 2024Review
- Melatonin and bone-related diseases: an updated mechanistic overview of current evidence and future prospects.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2023Review
- Absence of melatonin during development impairs craniofacial and dental onset in rats.Clinical oral investigations · 2023Article
- Small-molecule amines: a big role in the regulation of bone homeostasis.Bone research · 2023Review
- Effect of Ishophloroglucin A Isolated fromMarine drugs · 2023Article
- SP7: from Bone Development to Skeletal Disease.Current osteoporosis reports · 2023Review
- Daytime administration of melatonin has better protective effects on bone loss in ovariectomized rats.Journal of orthopaedic surgery and research · 2023Article
- Bone morphogenetic protein 4 is involved in cadmium-associated bone damage.Toxicological sciences : an official journal of the Society of Toxicology · 2023Article
- Melatonin Protects Bone Microarchitecture against Deterioration due to High-Fat Diet-Induced Obesity.Journal of bone metabolism · 2023Article
- Does Melatonin Associated with Nanostructured Calcium Phosphate Improve Alveolar Bone Repair?Medicina (Kaunas, Lithuania) · 2022Article
- Recent advances on small molecules in osteogenic differentiation of stem cells and the underlying signaling pathways.Stem cell research & therapy · 2022Review
- Influence of melatonin associated with the Bio-Gide® membrane on osteoblast activity: an in vitro Study.Acta odontologica latinoamericana : AOL · 2022Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the biological role of melatonin in osteogenic differentiation has been suggested, the mechanism of osteoblast differentiation remains unclear. Thus, the present study investigated the underlying molecular mechanisms based on osteoblast-specific transcription factors. We found that melatonin enhanced BMP-4-induced osteogenic differentiation and increased the expression of osteogenic markers, especially Osterix, which is an essential transcription factor for the differentiation of preosteoblasts into mature osteoblasts in the late stage of osteoblast differentiation. Melatonin treatment increased the expression of Osterix during osteoblast differentiation and stabilized its expression by the inhibition of ubiquitin-proteasome-mediated degradation of Osterix, leading to up-regulated Osterix transcriptional activity on the osteogenic promoter and promoting alkaline phosphatase activity and bone mineralization. Furthermore, treatment with protein kinase A (PKA) inhibitor H89 and protein kinase C (PKC) inhibitor Go6976 blocked the melatonin-induced transcriptional activity and phosphorylation of Osterix, indicating that melatonin regulates Osterix expression via the PKA and PKC signaling pathways. Overall, these findings suggest that melatonin directly regulates the late stage of osteoblast differentiation by enhancing Osterix expression; this provides further evidence of melatonin as a potent agent for treating osteoporosis.
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