Evidence map›Paper›PMID 28720201›Full record

ArticleJournal of biomechanics2017

Exposure to buffer solution alters tendon hydration and mechanics.

Babak N Safa, Kyle D Meadows, Spencer E Szczesny, Dawn M Elliott

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomechanics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
3.3field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 44 citations in OpenAlex.

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  6. Guidelines for ex vivo mechanical testing of tendon.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2023
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Babak N SafaDepartment of Mechanical Engineering, University of Delaware, Newark, DE, United States; Department of Biomedical Engineering, University of Delaware, Newark, DE, United States.
Kyle D MeadowsDepartment of Biomedical Engineering, University of Delaware, Newark, DE, United States.
Spencer E SzczesnyDepartment of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA, United States.
Dawn M ElliottDepartment of Mechanical Engineering, University of Delaware, Newark, DE, United States; Department of Biomedical Engineering, University of Delaware, Newark, DE, United States. Electronic address: delliott@udel.edu.
University of Delaware · USUniversity of Pennsylvania · US

Funding

Multi-scale biomechanics of engineered and native fibrous load-bearing tissueR01EB002425 · NIBIB · UNIVERSITY OF PENNSYLVANIA · PI ELLIOTT, DAWN M, MAUCK, ROBERT L · 2003 to 2017
$4.1M
NIBIB NIH HHS R01 EB002425
6 · The paper itself

Abstract

A buffer solution is often used to maintain tissue hydration during mechanical testing. The most commonly used buffer solution is a physiological concentration of phosphate buffered saline (PBS); however, PBS increases the tissue's water content and decreases its tensile stiffness. In addition, solutes from the buffer can diffuse into the tissue and interact with its structure and mechanics. These bathing solution effects can confound the outcome and interpretation of mechanical tests. Potential bathing solution artifacts, including solute diffusion, and their effect on mechanical properties, are not well understood. The objective of this study was to measure the effects of long-term exposure of rat tail tendon fascicles to several concentrations (0.9-25%) of NaCl, sucrose, polyethylene glycol (PEG), and SPEG (NaCl+PEG) solutions on water content, solute diffusion, and mechanical properties. We found that with an increase in solute concentration the apparent water content decreased for all solution types. Solutes diffused into the tissue for NaCl and sucrose, however, no solute diffusion was observed for PEG or SPEG. The mechanical properties changed for both NaCl solutions, in particular after long-term (8h) incubation the modulus and equilibrium stress decreased compared to short-term (15min) for 25% NaCl, and the cross sectional area increased for 0.9% NaCl. However, the mechanical properties were unchanged for both PEG and SPEG except for minor alterations in stress relaxation parameters. This study shows that NaCl and sucrose buffer solutions are not suitable for long-term mechanical tests. We therefore propose using PEG or SPEG as alternative buffer solutions that after long-term incubation can maintain tissue hydration without solute diffusion and produce a consistent mechanical response.

Indexed as

AnimalsBiomechanical PhenomenaBuffersDose-Response Relationship, DrugMechanical PhenomenaPolyethylene GlycolsRatsSodium ChlorideSolutionsTendonsWaterBuffersPolyethylene GlycolsSodium ChlorideSolutionsWaterBuffer solutionStress relaxationTendonTendon mechanicsTension

Identifiers

PMID28720201
PMCPMC5659756
OpenAlexW2724554611

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.