ArticleThe American journal of tropical medicine and hygiene2017
Performance of Real-Time Polymerase Chain Reaction Assays for the Detection of 20 Gastrointestinal Parasites in Clinical Samples from Senegal.
Article in The American journal of tropical medicine and hygiene, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
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- Commercial Simplex and Multiplex PCR Assays for the Detection of Intestinal ParasitesMicroorganisms · 2021Article
- Acquisition of multidrug-resistant bacteria and encoding genes among French pilgrims during the 2017 and 2018 Hajj.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- Occurrence of Ten Protozoan Enteric Pathogens in Three Non-Human Primate Populations.Pathogens (Basel, Switzerland) · 2021Article
- Parasitic Infections in African Humans and Non-Human Primates.Pathogens (Basel, Switzerland) · 2020Article
- Amoebic toxic megacolon with poly-helminthic coinfection: Case presentation and review of intestinal polyparasitic infections.International journal of surgery case reports · 2020Article
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- A Case for Using Genomics and a Bioinformatics Pipeline to Develop Sensitive and Species-Specific PCR-Based Diagnostics for Soil-Transmitted Helminths.Frontiers in genetics · 2019Review
- Low prevalence of resistance genes in sheltered homeless population in Marseille, France, 2014-2018.Infection and drug resistance · 2019Article
- The Presence ofFrontiers in cellular and infection microbiology · 2019Article
- A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France.Epidemiology and infection · 2019Article
- Evaluation of two DNA extraction methods for the PCR-based detection of eukaryotic enteric pathogens in fecal samples.BMC research notes · 2018Article
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Authors and funding
15 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gastrointestinal parasite infections represent one of the biggest public health problems in the world. Therefore, appropriate innovative tools are needed for assessing interventions to control these infections. This study aims to compare the performance of real-time polymerase chain reaction (PCR) assays to microscopic examination for detection of intestinal parasites. A direct microscopic examination and stool concentration was performed on 98 stool samples from patients attending Senegalese hospitals. Negative microscopic control samples were also collected in Nice and Marseille (France). Species-specific primers/probes were used to detect 20 common gastrointestinal protozoans and helminths. Positive frequency and the sensitivity of each real-time PCR assay were compared with conventional microscopic examination. Real-time PCR was positive in 72 of 98 samples (73.5%), whereas microscopic examination was positive in 37 (37.7%) samples (
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