Evidence map›Paper›PMID 28718081›Full record

Trial reportJournal of assisted reproduction and genetics2017

Blastocyst utilization rates after continuous culture in two commercial single-step media: a prospective randomized study with sibling oocytes.

Ioannis A Sfontouris, Efstratios M Kolibianakis, George T Lainas, Christos A Venetis, George K Petsas, Basil C Tarlatzis, Tryfon G Lainas

Registry-linked trialOpen access · greenAbstract readRandomized Controlled Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Journal of assisted reproduction and genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02302638 (Embryo Development and Utilization Using Two Commercial Single-step Media), which is not on this map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT02302638 nacompletednot on this map

Embryo Development and Utilization Using Two Commercial Single-step Media

TypeinterventionalSponsorEugoniaRan2014 to 2015Enrolled43ConditionsInfertilityArmsembryo culture in different single-step media
3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 13 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 3 countries.

Ioannis A SfontourisEugonia Assisted Reproduction Unit, 7 Ventiri Street, 11528, Athens, Greece. sfontouris@gmail.com.
Efstratios M KolibianakisUnit for Human Reproduction, First Department of Obstetrics and Gynecology, Papageorgiou General Hospital, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece.
George T LainasEugonia Assisted Reproduction Unit, 7 Ventiri Street, 11528, Athens, Greece.
Christos A VenetisWomen's and Children's Health, St. George Hospital, University of New South Wales, Sydney, Australia.
George K PetsasEugonia Assisted Reproduction Unit, 7 Ventiri Street, 11528, Athens, Greece.
Basil C TarlatzisUnit for Human Reproduction, First Department of Obstetrics and Gynecology, Papageorgiou General Hospital, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Tryfon G LainasEugonia Assisted Reproduction Unit, 7 Ventiri Street, 11528, Athens, Greece.
Eugonia · GRPapageorgiou General Hospital · GRSt George Hospital · AUUniversity of Nottingham · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe aim of this study is to determine whether blastocyst utilization rates are different after continuous culture in two different commercial single-step media.

methodsThis is a paired randomized controlled trial with sibling oocytes conducted in infertility patients, aged ≤40 years with ≥10 oocytes retrieved assigned to blastocyst culture and transfer. Retrieved oocytes were randomly allocated to continuous culture in either Sage one-step medium (Origio) or Continuous Single Culture (CSC) medium (Irvine Scientific) without medium renewal up to day 5 post oocyte retrieval. Main outcome measure was the proportion of embryos suitable for clinical use (utilization rate).

resultsA total of 502 oocytes from 33 women were randomly allocated to continuous culture in either Sage one-step medium (n = 250) or CSC medium (n = 252). Fertilization was performed by either in vitro fertilization or intracytoplasmic sperm injection, and embryo transfers were performed on day 5. Two patients had all blastocysts frozen due to the occurrence of severe ovarian hyperstimulation syndrome. Fertilization and cleavage rates, as well as embryo quality on day 3, were similar in the two media. Blastocyst utilization rates (%, 95% CI) [55.4% (46.4-64.1) vs 54.7% (44.9-64.6), p = 0.717], blastocyst formation rates [53.6% (44.6-62.5) vs 51.9 (42.2-61.6), p = 0.755], and proportion of good quality blastocysts [36.8% (28.1-45.4) vs 36.1% (27.2-45.0), p = 0.850] were similar in Sage one-step and CSC media, respectively.

conclusionsContinuous culture of embryos in Sage one-step and CSC media is associated with similar blastocyst development and utilization rates. Both single-step media appear to provide adequate support during in vitro preimplantation embryo development. Whether these observations are also valid for other continuous single medium protocols remains to be determined. CLINICAL TRIAL REGISTRATION NUMBER: NCT02302638.

Indexed as

Culture MediaAdultBlastocystEmbryo Culture TechniquesEmbryo TransferFemaleFertilization in VitroHumansLive BirthOocyte RetrievalOocytesPregnancySiblingsSperm Injections, IntracytoplasmicCulture MediaBlastocyst formationContinuous cultureEmbryo cultureSingle stepUtilization rate

Identifiers

PMID28718081
PMCPMC5633582
OpenAlexW2735626591

What OpenQuestion holds

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.