ArticleJournal of biomedical optics2017
Quantifying three-dimensional rodent retina vascular development using optical tissue clearing and light-sheet microscopy.
Article in Journal of biomedical optics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Advances in Optical Tissue Clearing for Three-Dimensional Ocular Imaging and Analysis.Journal of ophthalmology · 2026Review
- Microvascular Regeneration and Perfusion of Partially Decellularized Tracheal Grafts.The Laryngoscope · 2025Article
- Collagen crosslinking-induced corneal morphological changes: a three-dimensional light sheet Microscopy-based evaluation.Scientific reports · 2024Article
- Model based optimization for refractive index mismatched light sheet imaging.Optics express · 2024Article
- Semi-automated protocol to quantify and characterize fluorescent three-dimensional vascular images.PloS one · 2024Article
- Article
- Three-dimensional characterization of developing and adult ocular vasculature in mice using in toto clearing.Communications biology · 2022Article
- Dysfunctional cGMP Signaling Leads to Age-Related Retinal Vascular Alterations and Astrocyte Remodeling in Mice.International journal of molecular sciences · 2022Article
- Illumination angle correction during image acquisition in light-sheet fluorescence microscopy using deep learning.Biomedical optics express · 2022Article
- Age dependence of retinal vascular plexus attenuation in the triple transgenic mouse model of Alzheimer's disease.Experimental eye research · 2022Article
- Light-Sheet Microscopy of the Optic Nerve Reveals Axonal Degeneration and Microglial Activation in NMDA-Induced Retinal Injury.EC ophthalmology · 2021Article
- InVision: An optimized tissue clearing approach for three-dimensional imaging and analysis of intact rodent eyes.iScience · 2021Article
- Deep learning-based autofocus method enhances image quality in light-sheet fluorescence microscopy.Biomedical optics express · 2021Article
- Conventional histomorphometry and fast free of acrylamide clearing tissue (FACT) visualization of sciatic nerve in chicken (Veterinary research forum : an international quarterly journal · 2021Article
- Three-dimensional Imaging Coupled with Topological Quantification Uncovers Retinal Vascular Plexuses Undergoing Obliteration.Theranostics · 2021Article
- High resolution three-dimensional imaging of the ocular surface and intact eyeball using tissue clearing and light sheet microscopy.The ocular surface · 2020Article
- Clearing techniques for visualizing the nervous system in development, injury, and disease.Journal of neuroscience methods · 2020Review
- Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy.eLife · 2020Article
- FACT or PACT: A Comparison between Free-Acrylamide and Acrylamide-Based Passive Sodium Dodecyl Sulfate Tissue Clearing for whole Tissue Imaging.Cell journal · 2019Article
- Multiscale light-sheet for rapid imaging of cardiopulmonary system.JCI insight · 2018Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Retinal vasculature develops in a highly orchestrated three-dimensional (3-D) sequence. The stages of retinal vascularization are highly susceptible to oxygen perturbations. We demonstrate that optical tissue clearing of intact rat retinas and light-sheet microscopy provides rapid 3-D characterization of vascular complexity during retinal development. Compared with flat mount preparations that dissect the retina and primarily image the outermost vascular layers, intact cleared retinas imaged using light-sheet fluorescence microscopy display changes in the 3-D retinal vasculature rapidly without the need for point scanning techniques. Using a severe model of retinal vascular disruption, we demonstrate that a simple metric based on Sholl analysis captures the vascular changes observed during retinal development in 3-D. Taken together, these results provide a methodology for rapidly quantifying the 3-D development of the entire rodent retinal vasculature.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.