ArticleBiomedical optics express2017
Chemical reactivation of resin-embedded pHuji adds red for simultaneous two-color imaging with EGFP.
Article in Biomedical optics express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 9 citations in OpenAlex.
- Improved clearing method contributes to deep imaging of plant organs.Communications biology · 2022Article
- Cross-Streams Through the Ventral Posteromedial Thalamic Nucleus to Convey Vibrissal Information.Frontiers in neuroanatomy · 2021Article
- Pinpointing Morphology and Projection of Excitatory Neurons in Mouse Visual Cortex.Frontiers in neuroscience · 2019Article
- Penetration model for chemical reactivation for resin-embedded green fluorescent protein imaging.Journal of biomedical optics · 2018Article
- Reconstruction of Intratelencephalic Neurons in the Mouse Secondary Motor Cortex Reveals the Diverse Projection Patterns of Single Neurons.Frontiers in neuroanatomy · 2018Article
- Simultaneous Acquisition of Multicolor Information From Neural Circuits in Resin-Embedded Samples.Frontiers in neuroscience · 2018Article
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Authors and funding
15 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pH-sensitive fluorescent proteins enabling chemical reactivation in resin are useful tools for fluorescence microimaging. EGFP or EYFP is good for such applications. For simultaneous two-color imaging, a suitable red fluorescent protein is an urgent need. Here a pH-sensitive red fluorescent protein, pHuji, is selected and verified to remain pH-sensitive in HM20 resin. We observe 183% fluorescence intensity of pHuji in resin-embeded mouse brain and 29.08-fold fluorescence intensity of reactivated pHuji compared to the quenched state. pHuji and EGFP can be quenched and chemically reactivated simultaneously in resin, thus enabling simultaneous two-color micro-optical sectioning tomography of resin-embedded mouse brain. This method may greatly facilitate the visualization of neuronal morphology and neural circuits to promote understanding of the structure and function of the brain.
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