ArticleBiomedical optics express2017
Measuring the effect of a Western diet on liver tissue architecture by FLIM autofluorescence and harmonic generation microscopy.
Article in Biomedical optics express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Multimodal Phasor Analysis for Digital Pathology: Quantitative Characterization of Liver Iron Overload.Chemical & biomedical imaging · 2026Article
- Overcoming photon and spatiotemporal sparsity in fluorescence lifetime imaging with SparseFLIM.Communications biology · 2024Article
- Fluorescence Lifetime Multiplexing (FLEX) for simultaneous high dimensional spatial biology in 3D.Communications biology · 2024Article
- Altered metabolism and DAM-signatures in female brains and microglia with aging.Brain research · 2024Article
- Nascent shifts in renal cellular metabolism, structure, and function due to chronic empagliflozin in prediabetic mice.American journal of physiology. Cell physiology · 2024Article
- Altered Metabolism and DAM-signatures in Female Brains and Microglia with Aging.bioRxiv : the preprint server for biology · 2023Article
- Fibrosis quantification using multiphoton excitation imaging of picrosirius red stained cardiac tissue.Research square · 2023Article
- Riluzole Suppresses Growth and Enhances Response to Endocrine Therapy in ER+ Breast Cancer.Journal of the Endocrine Society · 2023Article
- Label-free fluorescence microscopy: revisiting the opportunities with autofluorescent molecules and harmonic generations as biosensors and biomarkers for quantitative biology.Biophysical reviews · 2023Review
- Label-Free Imaging Techniques to Evaluate Metabolic Changes Caused by Toxic Liver Injury in PCLS.International journal of molecular sciences · 2023Article
- Fatty Acids and Bilirubin as Intrinsic Autofluorescence Serum Biomarkers of Drug Action in a Rat Model of Liver Ischemia and Reperfusion.Molecules (Basel, Switzerland) · 2023Article
- Quantitative melanoma diagnosis using spectral phasor analysis of hyperspectral imaging from label-free slices.Frontiers in oncology · 2023Article
- Linear Combination Properties of the Phasor Space in Fluorescence Imaging.Sensors (Basel, Switzerland) · 2022Review
- Oral N-acetylcysteine decreases IFN-γ production and ameliorates ischemia-reperfusion injury in steatotic livers.Frontiers in immunology · 2022Article
- Type 1 Innate Lymphoid Cells Are Proinflammatory Effector Cells in Ischemia-Reperfusion Injury of Steatotic Livers.Frontiers in immunology · 2022Article
- Phasor-based image segmentation: machine learning clustering techniques.Biomedical optics express · 2021Article
- LAURDAN since Weber: The Quest for Visualizing Membrane Heterogeneity.Accounts of chemical research · 2021Article
- Reduction of fibrosis and immune suppressive cells in ErbB2-dependent tumorigenesis by an LXR agonist.PloS one · 2021Article
- Blind Resolution of Lifetime Components in Individual Pixels of Fluorescence Lifetime Images Using the Phasor Approach.The journal of physical chemistry. B · 2020Article
- Phasor approach to autofluorescence lifetime imaging FLIM can be a quantitative biomarker of chronic renal parenchymal injury.Kidney international · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
Abstract
The phasor approach to auto-fluorescence lifetime imaging was used to identify and characterize a long lifetime species (LLS) (~7.8 ns) in livers of mice fed with a Western diet. The size of the areas containing this LLS species depends on the type of diet and the size distribution shows Western diet has much larger LLS sizes. Combination of third harmonic generation images with FLIM identified the LLS species with fat droplets and the droplet size distribution was estimated. Second harmonic generation microscopy combined with phasor FLIM shows that there is an increase in fibrosis with a Western diet. A new decomposition in three components of the phasor plot shows that a Western diet is correlated with a higher fraction of free NADH, signifying more reducing condition and more glycolytic condition. Multiparametric analysis of phasor distribution shows that from the distribution of phasor points, a Western diet fed versus a low fat diet fed samples of mice livers can be separated. The phasor approach for the analysis of FLIM images of autofluorescence in liver specimens can result in discovery of new fluorescent species and then these new fluorescent species can help assess tissue architecture. Finally integrating FLIM and second and third harmonic analysis provides a measure of the advancement of fibrosis as an effect of diet.
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