ArticleBiomedical optics express2017
Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate.
Article in Biomedical optics express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- A fluorescence lifetime separation approach for FLIM live-cell imaging.Journal of microscopy · 2026Article
- Open-source modular field-programmable gate array system for two-photon mesoscope enabling multiarea, multidepth neural activity recording and lifetime imaging.Neurophotonics · 2026Article
- Carbon dots as probes in FLIM:a review of applications and advances in cellular imaging.RSC advances · 2025Review
- Multispectral laser-scanning pulse-sampling fluorescence lifetime system for large-scale tissue imaging.Optics letters · 2025Article
- Single-photon peak event detection (SPEED): a computational method for fast photon counting in fluorescence lifetime imaging microscopy.Optics express · 2021Article
- Real-time pixelwise phasor analysis for video-rate two-photon fluorescence lifetime imaging microscopy.Biomedical optics express · 2021Article
- Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation.Scientific reports · 2020Article
- Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates.Nature communications · 2020Article
- Tracking metabolic dynamics of apoptosis with high-speed two-photon fluorescence lifetime imaging microscopy.Biomedical optics express · 2019Article
- Widefield fluorescence lifetime imaging of protoporphyrin IX for fluorescence-guided neurosurgery: An ex vivo feasibility study.Journal of biophotonics · 2019Article
- Modeling cardiac complexity: Advancements in myocardial models and analytical techniques for physiological investigation and therapeutic developmentAPL bioengineering · 2019Review
- Optical microscopy approaches angstrom precision, in 3D!Light, science & applications · 2019Article
- Wavelength agile multi-photon microscopy with a fiber amplified diode laser.Biomedical optics express · 2018Article
- High-speed imaging of transient metabolic dynamics using two-photon fluorescence lifetime imaging microscopy.Optica · 2018Article
- High-resolution retinal swept source optical coherence tomography with an ultra-wideband Fourier-domain mode-locked laser at MHz A-scan rates.Biomedical optics express · 2018Article
Corrections and comments
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Authors and funding
7 authors.
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Abstract
Two-photon-excited fluorescence lifetime imaging microscopy (FLIM) is a chemically specific 3-D sensing modality providing valuable information about the microstructure, composition and function of a sample. However, a more widespread application of this technique is hindered by the need for a sophisticated ultra-short pulse laser source and by speed limitations of current FLIM detection systems. To overcome these limitations, we combined a robust sub-nanosecond fiber laser as the excitation source with high analog bandwidth detection. Due to the long pulse length in our configuration, more fluorescence photons are generated per pulse, which allows us to derive the lifetime with a single excitation pulse only. In this paper, we show high quality FLIM images acquired at a pixel rate of 1 MHz. This approach is a promising candidate for an easy-to-use and benchtop FLIM system to make this technique available to a wider research community.
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