ArticleChemical science2015
HOCl can appear in the mitochondria of macrophages during bacterial infection as revealed by a sensitive mitochondrial-targeting fluorescent probe.
Article in Chemical science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 238 citations in OpenAlex.
- Leveraging Chlorination-Based Mechanism for Resolving Subcellular Hypochlorous Acid.bioRxiv : the preprint server for biology · 2024Article
- An Anthracene Carboxamide-Based Fluorescent Probe for Rapid and Sensitive Detection of Mitochondrial Hypochlorite in Living Cells.Biosensors · 2023Article
- Activatable fluorescent probes for imaging and diagnosis of rheumatoid arthritis.Military Medical Research · 2023Review
- A Ratiometric Fluorescent Probe for Hypochlorite and Lipid Droplets to Monitor Oxidative Stress.Biosensors · 2023Article
- A novel mitochondria-targetable NIR fluorescent probe for monitoring intracellular hypobromous acid levels.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2022Article
- Rational construction of deep-red fluorescent probe for rapid detection of HClO and its application in bioimaging and paper-based sensing.Analytical and bioanalytical chemistry · 2022Article
- A lysosome-targetable fluorescent probe based on HClO-mediated cyclization reaction for imaging of hypochlorous acid.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2022Article
- Recent progress in developing fluorescent probes for imaging cell metabolites.Biomedical materials (Bristol, England) · 2021Review
- A novel two-photon ratiometric fluorescent probe for imaging and sensing of BACE1 in different regions of AD mouse brain.Chemical science · 2020Article
- Fluorescent probes for organelle-targeted bioactive species imaging.Chemical science · 2019Review
- A novel highly specific and ultrasensitive fluorescent probe for monitoring hypochlorous acid and its application in live cells.RSC advances · 2019Article
- Photostable Ratiometric Pdot Probe for in Vitro and in Vivo Imaging of Hypochlorous Acid.Journal of the American Chemical Society · 2017Article
- A practical strategy to design and develop an isoform-specific fluorescent probe for a target enzyme: CYP1A1 as a case study.Chemical science · 2017Article
- Responsive hetero-organelle partition conferred fluorogenic sensing of mitochondrial depolarization.Chemical science · 2017Article
- Nebraska Red: a phosphinate-based near-infrared fluorophore scaffold for chemical biology applications.Chemical communications (Cambridge, England) · 2016Article
- Quantification of cysteine hydropersulfide with a ratiometric near-infrared fluorescent probe based on selenium-sulfur exchange reaction.Chemical science · 2016Article
- Pyroglutamate aminopeptidase 1 may be an indicator of cellular inflammatory response as revealed using a sensitive long-wavelength fluorescent probe.Chemical science · 2016Article
- A pillar[5]arene-based [2]rotaxane lights up mitochondria.Chemical science · 2016Article
- A dual-functional lanthanide nanoprobe for both living cell imaging and ICP-MS quantification of active protease.Chemical science · 2016Article
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Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Macrophages, important cells of the innate immune system, can produce abundant HOCl in the cytoplasm to fight against bacteria. Recent studies suggest that mitochondria in macrophages play a role in antibacterial responses. During bacterial infection, however, it is uncertain whether HOCl is present in the mitochondria, mainly because of the lack of a suitable research method. Herein, by developing a new mitochondrial-targeting fluorescent HOCl probe, combined with confocal fluorescence imaging, we show for the first time that HOCl can appear in the mitochondria of macrophages (Raw264.7 cells) during bacterial infection, as confirmed with non-phagocytic cells and inhibitors as control experiments. Moreover, the developed probe exhibits an accurate mitochondrial-targeting ability, a fast response, and high selectivity and sensitivity (detection limit 9 nM), and is thus expected to be employed for further revealing the biological function of subcellular mitochondria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.