Evidence map›Paper›PMID 28717423›Full record

ArticleJournal of extracellular vesicles2017

Contribution of neuroblastoma-derived exosomes to the production of pro-tumorigenic signals by bone marrow mesenchymal stromal cells.

Rie Nakata, Hiroyuki Shimada, G Esteban Fernandez, Rob Fanter, Muller Fabbri, Jemily Malvar, Pascale Zimmermann, Yves A DeClerck

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of extracellular vesicles, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed, 1 pooled it
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.

  1. Pooled it
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  20. Role of Energy Metabolism in the Progression of Neuroblastoma.International journal of molecular sciences · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Rie NakataThe Saban Research Institute of Children's Hospital Los Angeles, University of Southern California, Los Angeles, CA, USA.
Hiroyuki ShimadaThe Saban Research Institute of Children's Hospital Los Angeles, University of Southern California, Los Angeles, CA, USA.
G Esteban FernandezThe Saban Research Institute of Children's Hospital Los Angeles, University of Southern California, Los Angeles, CA, USA.
Rob FanterThe Saban Research Institute of Children's Hospital Los Angeles, University of Southern California, Los Angeles, CA, USA.ORCID 0000-0003-2981-4182
Muller FabbriThe Saban Research Institute of Children's Hospital Los Angeles, University of Southern California, Los Angeles, CA, USA.
Jemily MalvarThe Saban Research Institute of Children's Hospital Los Angeles, University of Southern California, Los Angeles, CA, USA.ORCID 0000-0002-5481-6780
Pascale ZimmermannCentre de Recherche en Cancérologie de Marseille (CRCM), Inserm, U1068-CNRS UMR7258, Aix-Marseille Université, Institut Paoli-Calmettes, Marseille, France.ORCID 0000-0001-8768-1790
Yves A DeClerckThe Saban Research Institute of Children's Hospital Los Angeles, University of Southern California, Los Angeles, CA, USA.ORCID 0000-0002-3688-0113
University of Southern California · USCentre National de la Recherche Scientifique · FR

Funding

Vascular endothelial cell integrins in angiogenesisP01CA081403 · NCI · CHILDREN'S HOSPITAL OF LOS ANGELES · PI SEEGER, ROBERT CHARLES · 2000 to 2014
$28.5M
Targeting S1PR1/JAK2/STAT3 Signaling Axis in EMDRU54CA163117 · NCI · CHILDREN'S HOSPITAL OF LOS ANGELES · PI DECLERCK, YVES A · 2011 to 2015
$2.7M
NCI NIH HHS P01 CA081403NCI NIH HHS U54 CA163117
6 · The paper itself

Abstract

The bone marrow (BM) niche is a microenvironment promoting survival, dormancy and therapeutic resistance in tumor cells. Central to this function are mesenchymal stromal cells (MSCs). Here, using neuroblastoma (NB) as a model, we demonstrate that NB cells release an extracellular vesicle (EVs) whose protein cargo is enriched in exosomal proteins but lacks cytokines and chemokines. Using three different purification methods, we then demonstrate that NB-derived exosomes were captured by MSCs and induced the production of pro-tumorigenic cytokines and chemokines, including interleukin-6 (IL-6), IL-8/CXCL8, vascular endothelial cell growth factor and monocyte-chemotactic protein-1, with exosomes prepared by size exclusion chromatography having the highest activity. We found no correlation between the IL-6 and IL-8/CXCL8 stimulatory activity of exosomes from eight NB cell lines and their origin, degree of MYCN amplification, drug resistance and disease status. We then demonstrate that the uptake of NB exosomes by MSCs was associated with a rapid increase in ERK1/2 and AKT activation, and that blocking ERK1/2 but not AKT activation inhibited the IL-6 and IL-8/CXCL8 production by MSCs without affecting exosome uptake. Thus, we describe a new mechanism by which NB cells induce in MSCs an inflammatory reaction that contributes to a favorable microenvironment in the BM.

Indexed as

cell–cell interactionExosomesextracellular-signal-regulated kinaseextracellular vesiclesmesenchymal stromal cellsneuroblastoma

Identifiers

PMID28717423
PMCPMC5505006
OpenAlexW2630728762

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.