Evidence map›Paper›PMID 28717421›Full record

ArticleJournal of extracellular vesicles2017

Isolation of membrane vesicles from prokaryotes: a technical and biological comparison reveals heterogeneity.

Priscila Dauros Singorenko, Vanessa Chang, Alana Whitcombe, Denis Simonov, Jiwon Hong, Anthony Phillips, Simon Swift, Cherie Blenkiron

Open access · goldAbstract read
In one paragraph

Article in Journal of extracellular vesicles, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.

0numbers the graph read from it
0cells of the map it votes in
71citing papers in PubMed
5.6field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

71 citing papers in PubMed, 122 citations in OpenAlex.

  1. Review
  2. Review
  3. Outer membrane vesicles fromMicrobiology spectrum · 2026
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  5. Review
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  7. Article
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  9. Article
  10. Review
  11. Review
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  13. Review
  14. Establishment of minimum protein standards forMycobacteria (London, England) · 2025
    Article
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  16. Review
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  18. Article
  19. Journal of bacteriology · 2024
    Article
  20. Article

11 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Priscila Dauros SingorenkoDepartment of Molecular Medicine and Pathology, The University of Auckland, Auckland, New Zealand.
Vanessa ChangDepartment of Molecular Medicine and Pathology, The University of Auckland, Auckland, New Zealand.
Alana WhitcombeDepartment of Molecular Medicine and Pathology, The University of Auckland, Auckland, New Zealand.
Denis SimonovDepartment of Molecular Medicine and Pathology, The University of Auckland, Auckland, New Zealand.ORCID 0000-0001-9709-3554
Jiwon HongSchool of Biological Sciences, The University of Auckland, Auckland, New Zealand.ORCID 0000-0003-0936-3281
Anthony PhillipsDepartment of Molecular Medicine and Pathology, The University of Auckland, Auckland, New Zealand.
Simon SwiftDepartment of Molecular Medicine and Pathology, The University of Auckland, Auckland, New Zealand.
Cherie BlenkironDepartment of Molecular Medicine and Pathology, The University of Auckland, Auckland, New Zealand.ORCID 0000-0002-0217-3808
University of Auckland · NZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Prokaryotes release membrane vesicles (MVs) with direct roles in disease pathogenesis. MVs are heterogeneous when isolated from bacterial cultures so Density Gradient Centrifugation (DGC) is valuable for separation of MV subgroups from contaminating material. Here we report the technical variability and natural biological heterogeneity seen between DGC preparations of MVs for

Indexed as

Extracellular vesiclesmicrobenucleic acidsouter membrane vesiclespathogen

Identifiers

PMID28717421
PMCPMC5505020
OpenAlexW2623047193

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.