Evidence map›Paper›PMID 28716123›Full record

ArticleReproductive biology and endocrinology : RB&E2017

Differentiating mouse embryonic stem cells express markers of human endometrium.

P Parasar, C R Sacha, N Ng, E R McGuirk, S Chinthala, P Ozcan, J Lindsey, S Salas, M R Laufer, S A Missmer and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive biology and endocrinology : RB&E, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 17 citations in OpenAlex.

  1. Review
  2. The Role of Adenogenesis Factors in the Pathogenesis of Endometriosis.International journal of molecular sciences · 2025
    Review
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Review
  9. Article
  10. Single-cell RNA sequencing redefines the mesenchymal cell landscape of mouse endometrium.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021
    Article
  11. The Origin and Pathogenesis of Endometriosis.Annual review of pathology · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

P ParasarBoston Center for Endometriosis, Boston Children's and Brigham and Women's Hospitals, 333 and 221 Longwood Avenue, Boston, MA, 02115, USA.
C R SachaCenter for Infertility and Reproductive Surgery, Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital and Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA.
N NgCenter for Infertility and Reproductive Surgery, Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital and Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA.
E R McGuirkCenter for Infertility and Reproductive Surgery, Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital and Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA.
S ChinthalaCenter for Infertility and Reproductive Surgery, Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital and Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA.
P OzcanCenter for Infertility and Reproductive Surgery, Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital and Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA.
J LindseyCenter for Infertility and Reproductive Surgery, Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital and Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA.
S SalasCenter for Infertility and Reproductive Surgery, Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital and Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA.
M R LauferBoston Center for Endometriosis, Boston Children's and Brigham and Women's Hospitals, 333 and 221 Longwood Avenue, Boston, MA, 02115, USA.
S A MissmerBoston Center for Endometriosis, Boston Children's and Brigham and Women's Hospitals, 333 and 221 Longwood Avenue, Boston, MA, 02115, USA.
R M AnchanBoston Center for Endometriosis, Boston Children's and Brigham and Women's Hospitals, 333 and 221 Longwood Avenue, Boston, MA, 02115, USA. ranchan@bwh.harvard.edu.
Brigham and Women's Hospital · USHarvard University · USBoston Children's Hospital · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundModeling early endometrial differentiation is a crucial step towards understanding the divergent pathways between normal and ectopic endometrial development as seen in endometriosis.

methodsTo investigate these pathways, mouse embryonic stem cells (mESCs) and embryoid bodies (EBs) were differentiated in standard EB medium (EBM). Immunofluorescence (IF) staining and reverse-transcription polymerase chain reaction (RT-PCR) were used to detect expression of human endometrial cell markers on differentiating cells, which were sorted into distinct populations using fluorescence-activated cell sorting (FACS).

resultsA subpopulation (50%) of early differentiating mESCs expressed both glandular (CD9) and stromal (CD13) markers of human endometrium, suggestive of a novel endometrial precursor cell population. We further isolated a small population of endometrial mesenchymal stem cells, CD45-/CD146+/PDGFR-β+, from differentiating EBs, representing 0.7% of total cells. Finally, quantitative PCR demonstrated significantly amplified expression of transcription factors Hoxa10 and Foxa2 in CD13+ EBs isolated by FACS (p = 0.03).

conclusionsThese findings demonstrate that mESCs have the capacity to express human endometrial cell markers and demonstrate potential differentiation pathways of endometrial precursor and mesenchymal stem cells, providing an in vitro system to model early endometrial tissue development. This model represents a key step in elucidating the mechanisms of ectopic endometrial tissue growth. Such a system could enable the development of strategies to prevent endometriosis and identify approaches for non-invasive monitoring of disease progression.

Indexed as

Cell DifferentiationAnimalsBiomarkersCD13 AntigensCD146 AntigenCell LineEmbryoid BodiesEndometriosisEndometriumFemaleGene ExpressionHumansMiceMice, 129 StrainMice, Inbred C57BLMouse Embryonic Stem CellsBiomarkersCD13 AntigensCD146 AntigenReceptor, Platelet-Derived Growth Factor betaTetraspanin 29Animal modelDifferentiationEndometriosisEndometriumMouse embryonic stem cells

Identifiers

PMID28716123
PMCPMC5514487
OpenAlexW2734708277

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.