ArticleViruses2017
Synchronous Langat Virus Infection of Haemaphysalis longicornis Using Anal Pore Microinjection.
Article in Viruses, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The trial behind it
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Langat Virus Biology and Infection.Reviews in medical virology · 2026Review
- Deciphering the phosphorylation-based regulatory strategies of Haemaphysalis longicornis in heat stress.Parasites & vectors · 2025Article
- Composition of the Midgut Microbiota Structure ofAnimals : an open access journal from MDPI · 2024Article
- Article
- Horizontal and Vertical Transmission of Powassan Virus by the Invasive Asian Longhorned Tick,Frontiers in cellular and infection microbiology · 2022Article
- An Experimental Murine Model to Study Acquisition Dynamics of Tick-Borne Langat Virus inFrontiers in microbiology · 2022Article
- An efficient microinjection method to generate human anaplasmosis agent Anaplasma phagocytophilum-infected ticks.Scientific reports · 2020Article
- Effects of the essential oil from Cymbopogon citratus on mortality and morphology of the tick Haemaphysalis longicornis (Acari: Ixodidae).Experimental & applied acarology · 2020Article
- Vector competence of Haemaphysalis longicornis ticks for a Japanese isolate of the Thogoto virus.Scientific reports · 2018Article
- A Continuing Exploration of Tick-Virus Interactions Using Various Experimental Viral Infections of Hard Ticks.Frontiers in physiology · 2018Review
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tick-borne encephalitis virus (TBEV) serocomplex of flaviviruses consists of arboviruses that cause important diseases in animals and humans. The transmission of this group of viruses is commonly associated with tick species such as Ixodes spp., Dermacentor spp., and Hyalomma spp. In the case of Haemaphysalis longicornis, the detection and isolation of flaviviruses have been previously reported. However, studies showing survival dynamics of any tick-borne flavivirus in H. longicornis are still lacking. In this study, an anal pore microinjection method was used to infect adult H. longicornis with Langat virus (LGTV), a naturally attenuated member of the TBEV serocomplex. LGTV detection in ticks was done by real-time PCR, virus isolation, and indirect immunofluorescent antibody test. The maximum viral titer was recorded at 28 days post-inoculation, and midgut cells were shown to be the primary replication site. The tick can also harbor the virus for at least 120 days and can successfully transmit LGTV to susceptible mice as confirmed by detection of LGTV antibodies. However, no transovarial transmission was observed from the egg and larval samples. Taken together, our results highly suggest that anal pore microinjection can be an effective method in infecting adult H. longicornis, which can greatly assist in our efforts to study tick and virus interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.