ArticleCell proliferation2017
Curved microstructures promote osteogenesis of mesenchymal stem cells via the RhoA/ROCK pathway.
Article in Cell proliferation, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed.
- Topographical design principles for osteochondral tissue engineering.Bioactive materials · 2026Review
- Programmable Microcarriers for Stem Cell Therapy: Advanced Fabrication Strategies, Stem Cell Fate Regulatory Function and Biomedical Applications.International journal of molecular sciences · 2026Review
- Harnessing Advances in Bone Tissue Engineering for Design of Bone-on-Chip Systems.Advanced healthcare materials · 2026Review
- Biomimetic Nanoparticles for Bone Regeneration: Construction Strategies and Therapeutic Mechanisms.International journal of nanomedicine · 2026Review
- Erythropoietin-Stimulated Macrophage-Derived Extracellular Vesicles in Chitosan Hydrogel Rescue BMSCs Fate by Targeting EGFR to Alleviate Inflammatory Bone Loss in Periodontitis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Fish-derived biomaterials for tissue engineering: advances in scaffold fabrication and applications in regenerative medicine and cancer therapy.Theranostics · 2025Review
- Heraclenin promotes the osteogenic differentiation of bone marrow stromal cells by activating the RhoA/ROCK pathway.Histology and histopathology · 2024Article
- Effect of Porous Substrate Topographies on Cell Dynamics: A Computational Study.ACS biomaterials science & engineering · 2023Article
- Effect of graphene-oxide-modified osteon-like concentric microgrooved surface on the osteoclastic differentiation of macrophages.Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2023Article
- Integrating physicomechanical and biological strategies for BTE: biomaterials-induced osteogenic differentiation of MSCs.Theranostics · 2023Review
- Developing Porous Ortho- and Pyrophosphate-Containing Glass Microspheres; Structural and Cytocompatibility Characterisation.Bioengineering (Basel, Switzerland) · 2022Article
- Gaussian curvature-driven direction of cell fate toward osteogenesis with triply periodic minimal surface scaffolds.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Upregulation of Runt related transcription factor 1 (RUNX1) contributes to tendon-bone healing after anterior cruciate ligament reconstruction using bone mesenchymal stem cells.Journal of orthopaedic surgery and research · 2022Article
- The distinct roles of myosin IIA and IIB under compression stress in nucleus pulposus cells.Cell proliferation · 2021Article
- Effect of tetrahedral DNA nanostructures on proliferation and osteogenic differentiation of human periodontal ligament stem cells.Cell proliferation · 2019Article
- Matrix stiffness regulates arteriovenous differentiation of endothelial progenitor cells during vasculogenesis in nude mice.Cell proliferation · 2019Article
- Tetrahedral DNA nanostructures facilitate neural stem cell migration via activating RHOA/ROCK2 signalling pathway.Cell proliferation · 2018Article
- Substrate stiffness regulated migration and invasion ability of adenoid cystic carcinoma cells via RhoA/ROCK pathway.Cell proliferation · 2018Article
- Square prism micropillars improve osteogenicity of poly(methyl methacrylate) surfaces.Journal of materials science. Materials in medicine · 2018Article
- Stiffness regulates the proliferation and osteogenic/odontogenic differentiation of human dental pulp stem cells via the WNT signalling pathway.Cell proliferation · 2018Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesCells in the osteon reside in a curved space, accordingly, the curvature of the microenvironment is an important geometric feature in bone formation. However, it is not clear how curved microstructures affect cellular behaviour in bone tissue. MATERIALS AND
methodsRat primary bone marrow mesenchymal stem cells (BMSCs) on wavy microgrooves were exposed to PDMS substrates with various curvatures to investigate alterations in cellular morphology and osteogenic differentiation. Additionally, the expression levels of RhoA and its effectors were examined by immunofluorescence and quantitative PCR to determine the mechanisms of curvature-dependent osteogenic differentiation.
resultsWavy microgrooves caused dramatic nuclear distortion and cytoskeletal remodelling. We detected a noticeable increase in the expression of osteogenic-related genes in BMSCs in wavy microgroove groups, and the maximum expression was observed in the high curvature group. Moreover, immunofluorescent staining and quantitative RT-PCR results for RhoA and its effectors showed that the RhoA/ROCK signalling pathway is associated with curvature-dependent osteogenic differentiation.
conclusionsOur results illustrated that curved microstructures could promote BMSC differentiation to the osteogenic lineage, and the osteogenic effects of higher curvature are more obvious. Wavy microstructures could also influence the RhoA/ROCK pathway. Accordingly, curved microstructures may be useful in bone tissue engineering.
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