ArticleHistochemistry and cell biology2017
Quality control in diagnostic immunohistochemistry: integrated on-slide positive controls.
Article in Histochemistry and cell biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
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- "Green-path": planting seeds for environmental sustainability in diagnostic and research surgical pathology.Virchows Archiv : an international journal of pathology · 2026Review
- Validation of on-slide immunocytochemistry controls prepared from long-term preserved prefixed cell suspensions.Cancer cytopathology · 2026Article
- Automated paraffin embedding of tissue samples significantly reduces overall workflow time in routine pathology practice.Pathologica · 2026Article
- Review
- One protocol to rule them all: a pilot study to identify the best fixation and decalcification approach for bone marrow biopsy immunohistochemistry.Pathologica · 2025Article
- Be bold, start cold! cold formalin fixation of colorectal cancer specimens granted superior DNA and RNA quality for downstream molecular analysis.Histochemistry and cell biology · 2024Article
- Prognostic and predictive role of YKL-40 in anal squamous cell carcinoma: a serological and tissue-based analysis in a multicentric cohort.Frontiers in medicine · 2024Article
- Impact of formalin fixation on mismatch repair protein evaluation by immunohistochemistry.Virchows Archiv : an international journal of pathology · 2023Article
- Evaluating mismatch repair deficiency in colorectal cancer biopsy specimens.Histochemistry and cell biology · 2023Article
- Article
- HLA-G as a prognostic marker in stage II/III colorectal cancer: not quite there yet.Histochemistry and cell biology · 2022Article
- Methods for restoration of ki67 antigenicity in aged paraffin tissue blocks.Histochemistry and cell biology · 2021Article
- Development of novel quality control material based on CRISPR/Cas9 editing and xenografts for MLH1 protein deficiency testing.Journal of clinical laboratory analysis · 2021Article
- Characterization of soluble PD-L1 in pleural effusions of mesothelioma patients: potential implications in the immune response and prognosis.Journal of cancer research and clinical oncology · 2021Article
- Combining molecular and immunohistochemical analyses of key drivers in primary melanomas: interplay between germline and somatic variations.Oncotarget · 2018Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Standardization in immunohistochemistry is a priority in modern pathology and requires strict quality control. Cost containment has also become fundamental and auditing of all procedures must take into account both these principles. Positive controls must be routinely performed so that their positivity guarantees the appropriateness of the immunohistochemical procedure. The aim of this study is to develop a low cost (utilizing a punch biopsy-PB-tool) procedure to construct positive controls which can be integrated in the patient's tissue slide. Sixteen frequently used control blocks were selected and multiple cylindrical samples were obtained using a 5-mm diameter punch biopsy tool, separately re-embedding them in single blocks. For each diagnostic immunoreaction requiring a positive control, an integrated PB-control section (cut from the appropriate PB-control block) was added to the top right corner of the diagnostic slide before immunostaining. This integrated control technique permitted a saving of 4.75% in total direct lab costs and proved to be technically feasible and reliable. Our proposal is easy to perform and within the reach of all pathology labs, requires easily available tools, its application costs is less than using external paired controls and ensures that a specific control for each slide is always available.
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