ArticleFrontiers in neuroanatomy2017
CRISPR/Cas9-Mediated Zebrafish Knock-in as a Novel Strategy to Study Midbrain-Hindbrain Boundary Development.
Article in Frontiers in neuroanatomy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Advances in Fish Gene Editing.Animals : an open access journal from MDPI · 2026Review
- A multi-tiered mechanical mechanism shapes the early neural plate.Nature communications · 2025Article
- A robust knock-in approach using a minimal promoter and a minicircle.Developmental biology · 2024Article
- Efficient knock-in method enabling lineage tracing in zebrafish.Life science alliance · 2023Article
- Epigenetic Regulation of Endothelial Cell Lineages During Zebrafish Development-New Insights From Technical Advances.Frontiers in cell and developmental biology · 2022Review
- An Update on the Molecular Mechanism of the Vertebrate Isthmic Organizer Development in the Context of the Neuromeric Model.Frontiers in neuroanatomy · 2022Review
- Myocardial protective effect and transcriptome profiling of Naoxintong on cardiomyopathy in zebrafish.Chinese medicine · 2021Article
- Progress in Gene-Editing Technology of Zebrafish.Biomolecules · 2021Review
- Single-cell transcriptome analysis reveals thyrocyte diversity in the zebrafish thyroid gland.EMBO reports · 2020Article
- Dynamic properties of noise and Her6 levels are optimized by miR-9, allowing the decoding of the Her6 oscillator.The EMBO journal · 2020Article
- Expression Patterns of Inducible Cre Recombinase Driven by Differential Astrocyte-Specific Promoters in Transgenic Mouse Lines.Neuroscience bulletin · 2020Article
- Single cell sequencing of radial glia progeny reveals the diversity of newborn neurons in the adult zebrafish brain.Development (Cambridge, England) · 2020Article
- Modeling Lysosomal Storage Diseases in the Zebrafish.Frontiers in molecular biosciences · 2020Review
- Polyacrylamide Bead Sensors for in vivo Quantification of Cell-Scale Stress in Zebrafish Development.Scientific reports · 2019Article
- A novel conserved enhancer at zebrafish zic3 and zic6 loci drives neural expression.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Article
- Zebrafish Models of Neurodevelopmental Disorders: Limitations and Benefits of Current Tools and Techniques.International journal of molecular sciences · 2019Review
- Overview of the reporter genes and reporter mouse models.Animal models and experimental medicine · 2018Review
- In vivo genome editing via the HITI method as a tool for gene therapy.Journal of human genetics · 2018Review
- Bloody Zebrafish: Novel Methods in Normal and Malignant Hematopoiesis.Frontiers in cell and developmental biology · 2018Article
- Overlapping Distribution of Orexin and Endocannabinoid Receptors and Their Functional Interaction in the Brain of Adult Zebrafish.Frontiers in neuroanatomy · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The midbrain-hindbrain boundary (MHB) acts as an organizer and controls the fate of neighboring cells to develop into either mesencephalic (midbrain) or metencephalic (hindbrain) cells by secreting signaling molecules like Wnt1 and Fgf8. The zebrafish is an excellent vertebrate model for studying MHB development due to the ease of gene manipulation and the possibility of following cellular dynamics and morphogenetic processes using live imaging. Currently, only very few reporter and/or Cre-driver lines are available to study gene expression at the MHB, hampering the understanding of MHB development, and traditional transgenic technologies using promoter/enhancer fragments or bacterial artificial chromosome (BAC)-mediated transgenesis often do not faithfully recapitulate endogenous expression patterns. In contrast, CRISPR/Cas9-mediated genome editing technology now provides a great opportunity to efficiently knock-in or knock-out genes. We have generated four CRISPR/Cas9-based knock-in fluorescent reporter lines for two crucial genes involved in MHB development, namely
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.