Evidence map›Paper›PMID 28711957›Full record

ArticleJournal of biomolecular NMR2017

An improved protocol for amino acid type-selective isotope labeling in insect cells.

Yaqun Zhang, Hui Wei, Dianlin Xie, Deepa Calambur, Andrew Douglas, Mian Gao, Frank Marsilio, William J Metzler, Nicolas Szapiel, Ping Zhang and 3 more

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of biomolecular NMR, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 3 countries.

Yaqun ZhangDepartment of Protein Science, Bristol-Myers Squibb, Route 206 and Province Line Road, P.O. Box 4000, Princeton, NJ, 08543-4000, USA.
Hui WeiMass Spectrometry COEI, Bristol-Myers Squibb, Route 206 and Province Line Road, P.O. Box 4000, Princeton, NJ, 08543-4000, USA.
Dianlin XieDepartment of Protein Science, Bristol-Myers Squibb, Route 206 and Province Line Road, P.O. Box 4000, Princeton, NJ, 08543-4000, USA.
Deepa CalamburDepartment of Protein Science, Bristol-Myers Squibb, Route 206 and Province Line Road, P.O. Box 4000, Princeton, NJ, 08543-4000, USA.
Andrew DouglasDepartment of Protein Science, Bristol-Myers Squibb, Route 206 and Province Line Road, P.O. Box 4000, Princeton, NJ, 08543-4000, USA.
Mian GaoDepartment of Protein Science, Bristol-Myers Squibb, Route 206 and Province Line Road, P.O. Box 4000, Princeton, NJ, 08543-4000, USA.
Frank MarsilioDepartment of Protein Science, Bristol-Myers Squibb, Route 206 and Province Line Road, P.O. Box 4000, Princeton, NJ, 08543-4000, USA.
William J MetzlerLead Discovery and Optimization, Bristol-Myers Squibb, Route 206 and Province Line Road, P.O. Box 4000, Princeton, NJ, 08543-4000, USA.
Nicolas SzapielDepartment of Protein Science, Bristol-Myers Squibb, Route 206 and Province Line Road, P.O. Box 4000, Princeton, NJ, 08543-4000, USA.
Ping ZhangDepartment of Protein Science, Bristol-Myers Squibb, Route 206 and Province Line Road, P.O. Box 4000, Princeton, NJ, 08543-4000, USA.
Mark R WitmerDepartment of Protein Science, Bristol-Myers Squibb, Route 206 and Province Line Road, P.O. Box 4000, Princeton, NJ, 08543-4000, USA.
Luciano MuellerPCO DAS NMR, Bristol-Myers Squibb, Route 206 and Province Line Road, P.O. Box 4000, Princeton, NJ, 08543-4000, USA. luciano.mueller@bms.com.ORCID http://orcid.org/0000-0002-8956-2653
David HedinExpression Systems, LLC, 2537 Second Street, Davis, CA, 95618, USA.
Bristol-Myers Squibb (United States) · USBristol-Myers Squibb (Germany) · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An improved expression protocol is proposed for amino acid type-specific [

Indexed as

Amino AcidsAnimalsBaculoviridaeCarbon IsotopesCD4 AntigensCulture MediaFocal Adhesion Kinase 1Isotope LabelingNitrogen IsotopesNuclear Magnetic Resonance, BiomolecularProtein BiosynthesisSf9 CellsSpodopteraAmino AcidsCarbon IsotopesCD4 AntigensCulture MediaFocal Adhesion Kinase 1Nitrogen IsotopesBaculovirusHC-HSQCHNCO-TROSYInsect cell cultureLabelingMass spectrometryNMRYeastolate

Identifiers

PMID28711957
OpenAlexW2734845930

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.