ArticleBiophysical journal2017
The Effect of RNA Secondary Structure on the Self-Assembly of Viral Capsids.
Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Structural organization of HBV pgRNA genome driven by phase separation in capsid confinement.Nature communications · 2026Article
- From disorder to icosahedral symmetry: How conformation-switching subunits enable RNA virus assembly.Science advances · 2025Article
- Nonviral protein cages as tools to decipher and combat viral threats.Npj viruses · 2025Review
- Molecular Dynamics Characterization of the Free and Encapsidated RNA2 of CCMV with the oxRNA Model.Macromolecular rapid communications · 2024Article
- Stimulus-responsive assembly of nonviral nucleocapsids.Nature communications · 2024Article
- A tradeoff between enterovirus A71 particle stability and cell entry.Nature communications · 2023Article
- Article
- Relationships between RNA topology and nucleocapsid structure in a model icosahedral virus.Biophysical journal · 2021Article
- Structural 3D Domain Reconstruction of the RNA Genome from Viruses with Secondary Structure Models.Viruses · 2021Article
- Scaling relation between genome length and particle size of viruses provides insights into viral life history.iScience · 2021Article
- Physics of viral dynamics.Nature reviews. Physics · 2021Review
- Virus Assembly Pathways: Straying Away but Not Too Far.Small (Weinheim an der Bergstrasse, Germany) · 2020Article
- Physical virology: From virus self-assembly to particle mechanics.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2020Review
- Nonspherical Coacervate Shapes in an Enzyme-Driven Active System.Langmuir : the ACS journal of surfaces and colloids · 2020Article
- RNA Homopolymers Form Higher-Curvature Virus-like Particles Than Do Normal-Composition RNAs.Biophysical journal · 2019Article
- Spatiotemporal control of coacervate formation within liposomes.Nature communications · 2019Article
- Enzymatic Synthesis and Fractionation of Fluorescent PolyU RNAs.Bio-protocol · 2018Article
- A modelling paradigm for RNA virus assembly.Current opinion in virology · 2018Review
- Laboratory evolution of virus-like nucleocapsids from nonviral protein cages.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The effect of RNA stiffness on the self-assembly of virus particles.Journal of physics. Condensed matter : an Institute of Physics journal · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous work has shown that purified capsid protein (CP) of cowpea chlorotic mottle virus (CCMV) is capable of packaging both purified single-stranded RNA molecules of normal composition (comparable numbers of A, U, G, and C nucleobases) and of varying length and sequence, and anionic synthetic polymers such as polystyrene sulfonate. We find that CCMV CP is also capable of packaging polyU RNAs, which-unlike normal-composition RNAs-do not form secondary structures and which act as essentially structureless linear polymers. Following our canonical two-step assembly protocol, polyU RNAs ranging in length from 1000 to 9000 nucleotides (nt) are completely packaged. Surprisingly, negative-stain electron microscopy shows that all lengths of polyU are packaged into 22-nm-diameter particles despite the fact that CCMV CP prefers to form 28-nm-diameter (T = 3) particles when packaging normal-composition RNAs. PolyU RNAs >5000 nt in length are packaged into multiplet capsids, in which a single RNA molecule is shared between two or more 22-nm-diameter capsids, in analogy with the multiplets of 28-nm-diameter particles formed with normal-composition RNAs >5000 nt long. Experiments in which viral RNA competes for viral CP with polyUs of equal length show that polyU, despite its lack of secondary structure, is packaged more efficiently than viral RNA. These findings illustrate that the secondary structure of the RNA molecule-and its absence-plays an essential role in determining capsid structure during the self-assembly of CCMV-like particles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.