Evidence map›Paper›PMID 28710827›Full record

ArticleStem cells (Dayton, Ohio)2017

Comparison of Non-Coding RNAs in Exosomes and Functional Efficacy of Human Embryonic Stem Cell- versus Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Won Hee Lee, Wen-Yi Chen, Ning-Yi Shao, Dan Xiao, Xulei Qin, Natalie Baker, Hye Ryeong Bae, Tzu-Tang Wei, Yongjun Wang, Praveen Shukla and 4 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells (Dayton, Ohio), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed, 1 pooled it
3.1field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 2 countries.

Won Hee LeeStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA.
Wen-Yi ChenStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA.
Ning-Yi ShaoStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA.
Dan XiaoStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA.
Xulei QinStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA.
Natalie BakerStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA.
Hye Ryeong BaeStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA.
Tzu-Tang WeiStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA.
Yongjun WangStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA.
Praveen ShuklaStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA.
Haodi WuStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA.
Kazuki KodoStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA.
Sang-Ging OngStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA.
Joseph C WuStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, California, USA.ORCID 0000-0002-6068-8041
Cardiovascular Institute of the South · USCalifornia Institute for Regenerative Medicine · US

Funding

Elucidating Mechanism of Cardiac Fibrosis with Cell Village of Pooled Human iPSCsR01HL130020 · NHLBI · STANFORD UNIVERSITY · PI Ronglih Liao, MARK MERCOLA · 2016 to 2026
$7.3M
Modeling Susceptibility to Chemotherapy-Induced Cardiotoxicity Using Human iPSCsR01HL123968 · NHLBI · STANFORD UNIVERSITY · PI QUERTERMOUS, THOMAS, WU, JOSEPH C. · 2014 to 2021
$4.8M
Molecular Imaging of Cardiac Pluripotent Stem CellsR01HL133272 · NHLBI · STANFORD UNIVERSITY · PI WU, JOSEPH C. · 2016 to 2020
$2.4M
Assessment of Low-Dose Radiation Risk and Mechanisms of Individual RadiosensitivityR01HL132875 · NHLBI · STANFORD UNIVERSITY · PI WU, JOSEPH C. · 2016 to 2019
$1.9M
Cardiac Mitohormesis Protects Against Diabetic Cardiomyopathy Through MitophagyK99HL130416 · NHLBI · STANFORD UNIVERSITY · PI ONG, SANG GING · 2016 to 2017
$258k
Modeling Familial Dilated Cardiomyopathy Disease Mechanism Using Human iPS CellsF32HL115870 · NHLBI · STANFORD UNIVERSITY · PI CHEN, WEN YI · 2012 to 2014
$156k
NHLBI NIH HHS F32 HL115870NHLBI NIH HHS K99 HL130416NHLBI NIH HHS R01 HL123968NHLBI NIH HHS R01 HL130020NHLBI NIH HHS R01 HL132875NHLBI NIH HHS R01 HL133272
6 · The paper itself

Abstract

Both human embryonic stem cell-derived cardiomyocytes (ESC-CMs) and human induced pluripotent stem cell-derived CMs (iPSC-CMs) can serve as unlimited cell sources for cardiac regenerative therapy. However, the functional equivalency between human ESC-CMs and iPSC-CMs for cardiac regenerative therapy has not been demonstrated. Here, we performed a head-to-head comparison of ESC-CMs and iPSC-CMs in their ability to restore cardiac function in a rat myocardial infarction (MI) model as well as their exosomal secretome. Human ESCs and iPSCs were differentiated into CMs using small molecule inhibitors. Fluorescence-activated cell sorting analysis confirmed ∼85% and ∼83% of CMs differentiated from ESCs and iPSCs, respectively, were positive for cardiac troponin T. At a single-cell level, both cell types displayed similar calcium handling and electrophysiological properties, with gene expression comparable with the human fetal heart marked by striated sarcomeres. Sub-acute transplantation of ESC-CMs and iPSC-CMs into nude rats post-MI improved cardiac function, which was associated with increased expression of angiogenic genes in vitro following hypoxia. Profiling of exosomal microRNAs (miRs) and long non-coding RNAs (lncRNAs) revealed that both groups contain an identical repertoire of miRs and lncRNAs, including some that are known to be cardioprotective. We demonstrate that both ESC-CMs and iPSC-CMs can facilitate comparable cardiac repair. This is advantageous because, unlike allogeneic ESC-CMs used in therapy, autologous iPSC-CMs could potentially avoid immune rejection when used for cardiac cell transplantation in the future. Stem Cells 2017;35:2138-2149.

Indexed as

Cell DifferentiationCells, CulturedExosomesHuman Embryonic Stem CellsHumansInduced Pluripotent Stem CellsMyocytes, CardiacCell therapyEmbryonic stem cell-derived cardiomyocyteExosomesInduced pluripotent stem cell-derived cardiomyocyteStem cells

Identifiers

PMID28710827
PMCPMC5918285
OpenAlexW2735206626

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.