ArticlePlant biotechnology journal2018
Identification and functional analysis of two P450 enzymes of Gossypium hirsutum involved in DMNT and TMTT biosynthesis.
Article in Plant biotechnology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- A herbivore-inducible CYP82 catalyzes DMNT formation in Idesia polycarpa leaves.BMC plant biology · 2026Article
- Metabolic engineering strategies: utilizing different microbial strains and advanced technologies for the synthesis of high-valued terpenoids.Antonie van Leeuwenhoek · 2026Review
- Diterpenoids in medicinal plants: structure, distribution, biological activities, biosynthesis, and bioengineering prospects.Frontiers in plant science · 2026Review
- Cytochrome P450-Induced Backbone Rearrangements in Terpene Biosynthesis of Plants.Molecules (Basel, Switzerland) · 2025Review
- Glutathionylation-mediated degradation of a cap-binding protein enhances Arabidopsis resistance to Plutella xylostella.The Plant cell · 2025Article
- Advances in the Biosynthesis of Plant Terpenoids: Models, Mechanisms, and Applications.Plants (Basel, Switzerland) · 2025Review
- Engineering DMNT emission in cotton enhances direct and indirect defense against mirid bugs.Journal of advanced research · 2025Article
- Genome-wide identification and evolution-profiling analysis of tps gene family inFrontiers in plant science · 2025Article
- The plant terpenes DMNT and TMTT function as signaling compounds that attract Asian corn borer (Ostrinia furnacalis) to maize plants.Journal of integrative plant biology · 2024Article
- Identification and characterization of two P450 enzymes fromHorticulture research · 2024Article
- The microbial biosynthesis of noncanonical terpenoids.Applied microbiology and biotechnology · 2024Review
- Advances in the Biosynthesis of Terpenoids and Their Ecological Functions in Plant Resistance.International journal of molecular sciences · 2023Review
- Dynamic environmental interactions shaped by vegetative plant volatiles.Natural product reports · 2023Review
- Inactivation of RPX1 in Arabidopsis confers resistance to Plutella xylostella through the accumulation of the homoterpene DMNT.Plant, cell & environment · 2023Article
- Volatile DMNT directly protects plants againsteLife · 2021Article
- The role of volatiles in plant communication.The Plant journal : for cell and molecular biology · 2019Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The homoterpenes (3E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) and (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT) are major herbivore-induced plant volatiles that can attract predatory or parasitic arthropods to protect injured plants from herbivore attack. In this study, DMNT and TMTT were confirmed to be emitted from cotton (Gossypium hirsutum) plants infested with chewing caterpillars or sucking bugs. Two CYP genes (GhCYP82L1 and GhCYP82L2) involved in homoterpene biosynthesis in G. hirsutum were newly identified and characterized. Yeast recombinant expression and enzyme assays indicated that the two GhCYP82Ls are both responsible for the conversion of (E)-nerolidol to DMNT and (E,E)-geranyllinalool to TMTT. The two heterologously expressed proteins without cytochrome P450 reductase fail to convert the substrates to homoterpenes. Quantitative real-time PCR (qPCR) analysis suggested that the two GhCYP82L genes were significantly up-regulated in leaves and stems of G. hirsutum after herbivore attack. Subsequently, electroantennogram recordings showed that electroantennal responses of Microplitis mediator and Peristenus spretus to DMNT and TMTT were both dose dependent. Laboratory behavioural bioassays showed that females of both wasp species responded positively to DMNT and males and females of M. mediator could be attracted by TMTT. The results provide a better understanding of homoterpene biosynthesis in G. hirsutum and of the potential influence of homoterpenes on the behaviour of natural enemies, which lay a foundation to study genetically modified homoterpene biosynthesis and its possible application in agricultural pest control.
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