Evidence map›Paper›PMID 28710763›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

Zebra Tail Amplification: Accelerated Detection of Apoptotic Blunt-Ended DNA Breaks by In Situ Ligation.

Vladimir V Didenko

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Vladimir V DidenkoDepartment of Neurosurgery and Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA. vdidenko@bcm.edu.

Funding

Ligatable fluorescent probes using energy transfer for apoptosis detectionR01NS062842 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI DIDENKO, VLADIMIR V · 2009 to 2012
$994k
FRET probes for analysis of serpin-mediated apoptosis in focal brain ischemiaR01NS082553 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI DIDENKO, VLADIMIR V · 2014 to 2016
$946k
Semi-artificial nanomachines for detection of DNA damage and apoptosisR21EB006301 · NIBIB · BAYLOR COLLEGE OF MEDICINE · PI DIDENKO, VLADIMIR V · 2006 to 2008
$487k
Express assay for specific fluorescence imaging of apoptosis via phosphatase-assisted topoligation.R21EB022788 · NIBIB · BAYLOR COLLEGE OF MEDICINE · PI DIDENKO, VLADIMIR V · 2017 to 2018
$421k
Imaging mRNA turnover in P-bodies and its application to brain ischemiaR21NS101620 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI DIDENKO, VLADIMIR V · 2017 to 2018
$421k
Assay for dual detection of apoptosisR21NS064403 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI DIDENKO, VLADIMIR V · 2009 to 2010
$409k
APMV-topoisomerase assay for comprehensive assessment of efferocytosis in tissueR21AR066931 · NIAMS · BAYLOR COLLEGE OF MEDICINE · PI DIDENKO, VLADIMIR V · 2014 to 2015
$366k
Apoptosis driven by elastase inhibitor: new approach to detection and study.R21CA178365 · NCI · BAYLOR COLLEGE OF MEDICINE · PI DIDENKO, VLADIMIR V · 2013 to 2014
$357k
Detection of Topoisomerase II Mediated DNA Damage in StrokeR21NS054855 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI DIDENKO, VLADIMIR V · 2006 to 2007
$311k
NCI NIH HHS R21 CA178365NIAMS NIH HHS R21 AR066931NIBIB NIH HHS R21 EB006301NIBIB NIH HHS R21 EB022788NINDS NIH HHS R01 NS062842NINDS NIH HHS R01 NS082553NINDS NIH HHS R21 NS054855NINDS NIH HHS R21 NS064403NINDS NIH HHS R21 NS101620
6 · The paper itself

Abstract

In situ ligation (ISL) is a simple and specific technique for apoptosis labeling in tissue sections. In its most economical version ISL uses ordinary PCR-labeled DNA fragments as probes. In tissue sections these makeshift probes are ligated to apoptotic DNA breaks by T4 DNA ligase. The approach can selectively label 5'PO

Indexed as

ApoptosisDNA BreaksDNAHumansIn Situ Nick-End LabelingMolecular Probe TechniquesPolymerase Chain ReactionTissue FixationDNABlunt-ended DNA breaks labelingIn situ ligationIsothermal amplificationRapid apoptosis detectionSignal enhancementZebra tail amplification

Identifiers

PMID28710763
PMCPMC5673075

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.