ArticleThe Journal of cell biology1986
Microtubules and actin filaments are not critically involved in the biogenesis of epithelial cell surface polarity.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
50 citing papers in PubMed, 144 citations in OpenAlex.
- Review
- Genetically induced microtubule disruption in the mouse intestine impairs intracellular organization and transport.Molecular biology of the cell · 2018Article
- Migration of Nucleocapsids in Vesicular Stomatitis Virus-Infected Cells Is Dependent on both Microtubules and Actin Filaments.Journal of virology · 2016Article
- Simvastatin modulates cellular components in influenza A virus-infected cells.International journal of molecular medicine · 2014Article
- A Rab11- and microtubule-dependent mechanism for cytoplasmic transport of influenza A virus viral RNA.Journal of virology · 2011Article
- Integrin-linked kinase controls microtubule dynamics required for plasma membrane targeting of caveolae.Developmental cell · 2010Article
- Apical trafficking in epithelial cells: signals, clusters and motors.Journal of cell science · 2009Review
- Involvement of dynein and spectrin with early melanosome transport and melanosomal protein trafficking.The Journal of investigative dermatology · 2008Article
- Constitutive secretion of protease nexin-1 by glial cells and its regulation by G-protein-coupled receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- KIFC3, a microtubule minus end-directed motor for the apical transport of annexin XIIIb-associated Triton-insoluble membranes.The Journal of cell biology · 2001Article
- cdc42 regulates the exit of apical and basolateral proteins from the trans-Golgi network.The EMBO journal · 2001Article
- Actin filaments and microtubules are involved in different membrane traffic pathways that transport sphingolipids to the apical surface of polarized HepG2 cells.Molecular biology of the cell · 1998Article
- Host cell dependence of viral morphology.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Role of actin microfilaments in Black Creek Canal virus morphogenesis.Journal of virology · 1998Article
- Apiconuclear organization of microtubules does not specify protein delivery from the trans-Golgi network to different membrane domains in polarized epithelial cells.Molecular biology of the cell · 1998Article
- Myosin II is involved in the production of constitutive transport vesicles from the TGN.The Journal of cell biology · 1997Article
- Involvement of the mutated M protein in altered budding polarity of a pantropic mutant, F1-R, of Sendai virus.Journal of virology · 1996Article
- Regulation of cell surface polarity in renal epithelia.Pediatric nephrology (Berlin, Germany) · 1993Review
- Possible involvement of microtubule disruption in bipolar budding of a Sendai virus mutant, F1-R, in epithelial MDCK cells.Journal of virology · 1993Article
- Actin microfilaments play a critical role in endocytosis at the apical but not the basolateral surface of polarized epithelial cells.The Journal of cell biology · 1993Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We have studied the role of microtubules and actin filaments in the biogenesis of epithelial cell surface polarity, using influenza hemagglutinin and vesicular stomatitis G protein as model apical and basolateral proteins in infected Madin-Darby canine kidney cells. Addition of colchicine or nocodazole to confluent monolayers at concentrations sufficient to completely disassemble microtubules did not affect the asymmetric budding of influenza or vesicular stomatitis virus and only slightly reduced the typical asymmetric surface distribution of their envelope proteins, despite extensive cytoplasmic redistribution of the Golgi apparatus. Alteration of microtubular function by taxol or dissociation of actin filaments by cytochalasin D also failed to have a significant effect. Furthermore, neither colchicine nor cytochalasin D pretreatment blocked the ability of subconfluent Madin-Darby canine kidney cells to sustain polarized budding of influenza virus a few hours after attachment to the substrate. Our results indicate that domain-specific microtubule or actin filament "tracks" are not responsible for the vectorial delivery of apically or basolaterally directed transport vesicles. In conjunction with currently available evidence, they are compatible with a model in which receptors in the cytoplasmic aspect of apical or basolateral regions provide vectoriality to the transport of vesicles carrying plasma membrane proteins to their final surface localization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.