Evidence map›Paper›PMID 28709015›Full record

ArticleDNA repair2017

Human cells contain a factor that facilitates the DNA glycosylase-mediated excision of oxidized bases from occluded sites in nucleosomes.

R L Maher, C G Marsden, A M Averill, S S Wallace, J B Sweasy, D S Pederson

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in DNA repair, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 25 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Review
  5. Article
  6. Generation of Recombinant Nucleosomes Containing Site-Specific DNA Damage.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  7. Article
  8. Article
  9. Review
  10. Article
  11. Article
  12. Review
  13. Coevolution of body size and metabolic rate in vertebrates: a life-history perspective.Biological reviews of the Cambridge Philosophical Society · 2020
    Article
  14. Article
  15. Article
  16. Damage sensor role of UV-DDB during base excision repair.Nature structural & molecular biology · 2019
    Article
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

R L MaherDepartment of Microbiology and Molecular Genetics, and The Markey Center for Molecular Genetics, University of Vermont, Burlington, VT, 05405, USA.
C G MarsdenDepartment of Microbiology and Molecular Genetics, and The Markey Center for Molecular Genetics, University of Vermont, Burlington, VT, 05405, USA.
A M AverillDepartment of Microbiology and Molecular Genetics, and The Markey Center for Molecular Genetics, University of Vermont, Burlington, VT, 05405, USA.
S S WallaceDepartment of Microbiology and Molecular Genetics, and The Markey Center for Molecular Genetics, University of Vermont, Burlington, VT, 05405, USA.
J B SweasyDepartment of Microbiology and Molecular Genetics, and The Markey Center for Molecular Genetics, University of Vermont, Burlington, VT, 05405, USA; Departments of Therapeutic Radiology and Human Genetics, Yale University School of Medicine, New Haven, CT 06520, USA.
D S PedersonDepartment of Microbiology and Molecular Genetics, and The Markey Center for Molecular Genetics, University of Vermont, Burlington, VT, 05405, USA. Electronic address: david.pederson@uvm.edu.
University of Vermont · US

Funding

Yale Clinical and Translational Science Award (U Component)UL1TR001863 · NCATS · YALE UNIVERSITY · PI John H. Krystal, LUCILA OHNO-MACHADO · 2016 to 2026
$102.9M
SUBSTRATE SPECIFICITY OF THE BER OXIDATIVE DNA GLYCOSYLASESP01CA098993 · NCI · UNIVERSITY OF VERMONT &ST AGRIC COLLEGE · PI LEE, ANDREA J · 2004 to 2021
$25.9M
NCATS NIH HHS UL1 TR001863NCI NIH HHS P01 CA098993
6 · The paper itself

Abstract

Reactive oxygen species generate some 20,000 base lesions per human cell per day. The vast majority of these potentially mutagenic or cytotoxic lesions are subject to base excision repair (BER). Although chromatin remodelers have been shown to enhance the excision of oxidized bases from nucleosomes in vitro, it is not clear that they are recruited to and act at sites of BER in vivo. To test the hypothesis that cells possess factors that enhance BER in chromatin, we assessed the capacity of nuclear extracts from human cells to excise thymine glycol (Tg) lesions from exogenously added, model nucleosomes. The DNA glycosylase NTHL1 in these extracts was able to excise Tg from both naked DNA and sites in nucleosomes that earlier studies had shown to be sterically accessible. However, the same extracts were able to excise lesions from sterically-occluded sites in nucleosomes only after the addition of Mg

Indexed as

Chromatin Assembly and DisassemblyDNA DamageDNA RepairAdenosine TriphosphateCations, DivalentCell LineDeoxyribonuclease (Pyrimidine Dimer)DNAHumansMagnesiumNucleosomesThymineAdenosine TriphosphateCations, DivalentDeoxyribonuclease (Pyrimidine Dimer)DNAMagnesiumNTHL1 protein, humanNucleosomesThyminethymine glycolBase excision repair (BER)Chromatin remodelersNTHL1NucleosomesThymine glycol (Tg)

Identifiers

PMID28709015
PMCPMC5569575
OpenAlexW2726583977

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.