ArticleClinical oral investigations2018
The role of macrophage polarization on fibroblast behavior-an in vitro investigation on titanium surfaces.
Article in Clinical oral investigations, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
- The role of medium-rough implant surface topography on macrophages polarization in age-independent manner.BMC oral health · 2026Article
- Inducing macrophage M2-Type polarization via anodized anisotropic TiOBMC oral health · 2025Article
- Topography-based implants for bone regeneration: Design, biological mechanism, and therapeutics.Materials today. Bio · 2025Review
- Development of Zinc-Containing Chitosan/Gelatin Coatings with Immunomodulatory Effect for Soft Tissue Sealing around Dental Implants.Tissue engineering and regenerative medicine · 2025Article
- Roughness affects the response of human fibroblasts and macrophages to sandblasted abutments.Biomedical engineering online · 2024Article
- Effects of bone surface topography and chemistry on macrophage polarization.Scientific reports · 2024Article
- Mesoporous MOFs with ROS scavenging capacity for the alleviation of inflammation through inhibiting stimulator of interferon genes to promote diabetic wound healing.Journal of nanobiotechnology · 2024Article
- Laser-Modified Ti Surface Improves Paracrine Osteogenesis by Modulating the Expression of DKK1 in Osteoblasts.Journal of functional biomaterials · 2023Article
- Biomaterial scaffolds regulate macrophage activity to accelerate bone regeneration.Frontiers in bioengineering and biotechnology · 2023Review
- Recent Developments in Extracellular Matrix Remodeling for Fat Grafting.Frontiers in cell and developmental biology · 2021Review
- Multi-Scale Surface Treatments of Titanium Implants for Rapid Osseointegration: A Review.Nanomaterials (Basel, Switzerland) · 2020Review
- Niobium-Treated Titanium Implants with Improved Cellular and Molecular Activities at the Tissue-Implant Interface.Materials (Basel, Switzerland) · 2019Article
- Macrophage behavior and interplay with gingival fibroblasts cultured on six commercially available titanium, zirconium, and titanium-zirconium dental implants.Clinical oral investigations · 2019Article
- Free Radical Production, Inflammation and Apoptosis in Patients Treated With Titanium Mandibular Fixations-An Observational Study.Frontiers in immunology · 2019Observational
- Screening of biopolymeric materials for cardiovascular surgery toxicity-Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients.Toxicology reports · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
objectivesThis study investigated the effect of smooth and rough titanium surface topographies on macrophage polarization and their influence on gingival fibroblast behavior cultured on titanium surfaces. MATERIALS AND
methodsRAW 264.7 macrophages were seeded on smooth (pickled titanium (PT)) and rough Sand-blasted with Large grit particles followed by Acid-etching (SLA) titanium surfaces and first investigated for macrophage polarization towards tissue-inflammatory M1 macrophages or wound-healing M2 macrophages. Thereafter, culture media collected from macrophages on both surfaces were cultured with gingival fibroblasts seeded on their respective topographies. All experiments were performed in triplicate with three independent experiments.
resultsMacrophages seeded on SLA surfaces polarized towards tissue-inflammatory M1 macrophages at early time points. Immunofluorescent staining and RT-PCR analysis demonstrated higher levels of iNOS and gene expression of IL-1β, IL-6, and TNF-alpha on SLA surfaces at 3 days when compared to both tissue culture plastic (TCP) and PT surfaces (p < 0.001). Very little differences were found between smooth PT surfaces and TCP. Interestingly, proliferation assay (CCK-8) suggested that conditioned media (CM) from macrophages seeded on SLA surfaces drastically inhibited gingival fibroblast proliferation at 3 and 5 days (p < 0.001). Meanwhile, CM from macrophages cultured on SLA surfaces also significantly reduced collagen 1 synthesis on SLA surfaces at 14 days as assessed by immunofluorescent staining (p < 0.001).
conclusionThe results from this study demonstrate that the polarization of macrophages towards a pro-inflammatory (M1) phenotype on SLA surfaces may have a negative impact on gingival fibroblast behavior on titanium surfaces. Future strategies to better modulate macrophage polarization should be investigated to support a favorable immune response and encourage tissue integration. CLINICAL RELEVANCE: As SLA surfaces have a potential to shift macrophages towards tissue-inflammatory M1 macrophages, this might be a negative impact for soft tissue healing. Therefore, SLA surfaces should be kept within the bone, as when in contact with soft tissue, they are prone to support a lack of soft tissue integration leading to inflammation.
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