ArticleFrontiers in plant science2017
Identification of Optimal Reference Genes for Normalization of qPCR Analysis during Pepper Fruit Development.
Article in Frontiers in plant science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Transcriptome-Based Selection and Validation of Reference Genes for Gene Expression Analysis inGenes · 2026Article
- Optimizing reference gene selection for accurate gene expression analysis in plants.Plant signaling & behavior · 2025Review
- The Expression Profile of Genes Related to Carotenoid Biosynthesis in Pepper Under Abiotic Stress Reveals a Positive Correlation with Plant Tolerance.Life (Basel, Switzerland) · 2024Article
- Article
- Selection and verification of reliable internal reference genes in stem development of herbaceous peony (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Microscopic and metabolic investigations disclose the factors that lead to skin cracking in chili-type pepper fruit varieties.Horticulture research · 2023Article
- Biocontrol of Diseases Caused byJournal of fungi (Basel, Switzerland) · 2023Article
- Red LED Light Improves Pepper (International journal of molecular sciences · 2023Article
- Genome-wide characterization of ascorbate peroxidase gene family in pepper (Frontiers in plant science · 2023Article
- Evaluation of reference genes for transcript normalization inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Selection and validation of reference genes for qRT-PCR analysis during fruit ripening of red pitaya (Hylocereus polyrhizus).FEBS open bio · 2021Article
- Temporal expression patterns of fruit-specific α- EXPANSINS during cell expansion in bell pepper (Capsicum annuum L.).BMC plant biology · 2020Article
- Identification and evaluation of suitable reference genes for gene expression analysis in rubber tree leaf.Molecular biology reports · 2020Article
- Identification and evaluation of reference genes for reliable normalization of real-time quantitative PCR data in acerola fruit, leaf, and flower.Molecular biology reports · 2020Article
- Genome-Wide Identification and Characterization of the Mitochondrial Transcription Termination Factors (mTERFs) inInternational journal of molecular sciences · 2019Article
- Selection of reliable reference genes for quantitative RT-PCR in garlic under salt stress.PeerJ · 2019Article
- Selection of the optimal reference genes for expression analyses in different materials ofPlant methods · 2019Article
- Candidate Reference Genes Selection and Application for RT-qPCR Analysis in Kenaf with Cytoplasmic Male Sterility Background.Frontiers in plant science · 2017Article
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12 authors.
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Abstract
Due to its high sensitivity and reproducibility, quantitative real-time PCR (qPCR) is practiced as a useful research tool for targeted gene expression analysis. For qPCR operations, the normalization with suitable reference genes (RGs) is a crucial step that eventually determines the reliability of the obtained results. Although pepper is considered an ideal model plant for the study of non-climacteric fruit development, at present no specific RG have been developed or validated for the qPCR analyses of pepper fruit. Therefore, this study aimed to identify stably expressed genes for their potential use as RGs in pepper fruit studies. Initially, a total of 35 putative RGs were selected by mining the pepper transcriptome data sets derived from the PGP (Pepper Genome Platform) and PGD (Pepper Genome Database). Their expression stabilities were further measured in a set of pepper (
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