Evidence map›Paper›PMID 28706421›Full record

ArticleWorld journal of gastroenterology2017

Gastric cancer-derived heat shock protein-gp96 peptide complex enhances dendritic cell activation.

Wen-Wen Lu, Hong Zhang, You-Ming Li, Feng Ji

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of gastroenterology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 9 citations in OpenAlex.

  1. Pooled it
  2. Review
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  6. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Wen-Wen LuWen-Wen Lu, Department of Geriatrics, the First Affiliated Hospital of Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
Hong ZhangWen-Wen Lu, Department of Geriatrics, the First Affiliated Hospital of Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
You-Ming LiWen-Wen Lu, Department of Geriatrics, the First Affiliated Hospital of Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
Feng JiWen-Wen Lu, Department of Geriatrics, the First Affiliated Hospital of Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
First Affiliated Hospital Zhejiang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo investigate the role of heat shock protein (HSP)-glycoprotein (gp)96 in dendritic cells (DCs) and lymphocytes induction in gastric cancer (GC).

methodsHuman GC cell lines KATOIII, MKN-28 and SGC-7901 were infected with adenovirus gp96 at a multiplicity of infection of 100. gp96-GC antigen peptide complexes were purified. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, lactate dehydrogenase (LDH) release assay and enzyme-linked immunosorbent assay were used to determine allo-reactive T cell stimulation, natural killer (NK) cell activity and expression of cytokines (such as interleukin (IL)-10, IL-12, interferon (IFN)-γ and tumor necrosis factor (TNF)-α), respectively. Effect of cytotoxic T lymphocyte (CTL) on DCs incubated with HSP-gp96 was also evaluated by LDH release. All assays were performed in triplicate and the average values were reported. Comparison between groups was conducted using Student's

resultsT cells incubated with HSP-gp96 exhibited a marked increase in proliferation in a dose-dependent manner (

conclusionHSP-gp96 promotes T cell response, enhances DC antigen presentation and induces cytokine secretion, as well. HSP-gp96 has potential as immunotherapy for elimination of residual GC cells.

Indexed as

AdenoviridaeAntigen PresentationAntigens, NeoplasmCell Line, TumorCell ProliferationCoculture TechniquesCytokinesDendritic CellsEnzyme-Linked Immunosorbent AssayGenetic VectorsHeat-Shock ProteinsHumansKiller Cells, NaturalLymphocyte ActivationMembrane GlycoproteinsStomach NeoplasmsAntigens, NeoplasmCytokinesendoplasminHeat-Shock ProteinsMembrane GlycoproteinsCytotoxic T lymphocytesDendritic cellsGastric cancerHeat shock protein gp96Immunization therapyNatural killer cellsT lymphocytes

Identifiers

PMID28706421
PMCPMC5487502
OpenAlexW2647960909

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.