ArticleScientific reports2017
Patch clamp-assisted single neuron lipidomics.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Lipidomic profiling reveals age-dependent changes in plasma membrane lipids that affect neural stem cell aging.Science advances · 2026Article
- Miniaturized chromatography-based lipidomics methods towards single-cell analysis.Analytical and bioanalytical chemistry · 2026Review
- Characterization of the Lipidome of Neurons in Mouse Brain Nuclei Using Imaging Mass Spectrometry.Analytical chemistry · 2026Article
- High-Throughput Fluorescence-Guided Sequential Single-Cell MALDI-ICC Mass Spectrometry.Analytical chemistry · 2025Article
- Unravelling lipid heterogeneity: Advances in single-cell lipidomics in cellular metabolism and disease.BBA advances · 2025Review
- Lipidome atlas of the adult human brain.Nature communications · 2024Article
- Analytical Techniques for Single-Cell Biochemical Assays of Lipids.Annual review of biomedical engineering · 2023Review
- Article
- Secondary Ion Mass Spectrometry Imaging Reveals Changes in the Lipid Structure of the Plasma Membranes of Hippocampal Neurons following Drugs Affecting Neuronal Activity.ACS chemical neuroscience · 2021Article
- Acyl-CoA synthetases as regulators of brain phospholipid acyl-chain diversity.Prostaglandins, leukotrienes, and essential fatty acids · 2020Review
- Differential lipid composition and regulation along the hippocampal longitudinal axis.Translational psychiatry · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
Abstract
Our understanding of the physiological and pathological functions of brain lipids is limited by the inability to analyze these molecules at cellular resolution. Here, we present a method that enables the detection of lipids in identified single neurons from live mammalian brains. Neuronal cell bodies are captured from perfused mouse brain slices by patch clamping, and lipids are analyzed using an optimized nanoflow liquid chromatography/mass spectrometry protocol. In a first application of the method, we identified more than 40 lipid species from dentate gyrus granule cells and CA1 pyramidal neurons of the hippocampus. This survey revealed substantial lipid profile differences between neurons and whole brain tissue, as well as between resting and physiologically stimulated neurons. The results suggest that patch clamp-assisted single neuron lipidomics could be broadly applied to investigate neuronal lipid homeostasis in healthy and diseased brains.
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