ArticlePloS one2017
RNAi-based reverse genetics in the chelicerate model Tetranychus urticae: A comparative analysis of five methods for gene silencing.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 93 citations in OpenAlex.
- Interfering with DNA repair pathways to enhance CRISPR-Cas9-mediated homology-directed repair in a chelicerate genetic model.iScience · 2026Article
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- Identification of potential RNAi targets and analysis of dsRNA stability and intracellular transport in the underground pest Holotrichia parallela.Pest management science · 2026Article
- RNAi-mediated knockdown of the poultry red mite cathepsin D-1 impacts haemoglobin digestion.Parasites & vectors · 2026Article
- Dual roles of salivary proteins in feeding and silk fiber coating in the spider mite Tetranychus urticae.Communications biology · 2025Article
- Syntaxin-1A Silencing by RNAi Disrupts Growth and Reproduction in the Asian Citrus Psyllid,Insects · 2025Article
- Article
- Target gene selection for sprayable dsRNA-based biopesticide against Tetranychus urticae Koch (Acari: Tetranychidae).Pest management science · 2025Article
- Nanoparticle LDH enhances RNAi efficiency of dsRNA in piercing-sucking pests by promoting dsRNA stability and transport in plants.Journal of nanobiotechnology · 2024Article
- Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference inNanomaterials (Basel, Switzerland) · 2022Article
- Localized efficacy of environmental RNAi in Tetranychus urticae.Scientific reports · 2022Article
- Functional Role ofInsects · 2022Article
- β-Cyanoalanine synthase protects mites against Arabidopsis defenses.Plant physiology · 2022Article
- Trunk injection delivery of dsRNA for RNAi-based pest control in apple trees.Pest management science · 2022Article
- Identification of the fibroin of Stigmaeopsis nanjingensis by a nanocarrier-based transdermal dsRNA delivery system.Experimental & applied acarology · 2022Article
- Molecular characterization, expression, and function of Vitellogenin genes in Phytoseiulus persimilis.Experimental & applied acarology · 2022Article
- An RNA Interference Tool to Silence Genes inInternational journal of molecular sciences · 2022Article
- Circadian and Neuroendocrine Basis of Photoperiodism Controlling Diapause in Insects and Mites: A Review.Frontiers in physiology · 2022Review
- Double-Strand RNA (dsRNA) Delivery Methods in Insects: Diaphorina citri.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Methods for Delivery of dsRNAs for Agricultural Pest Control: The Case of Lepidopteran Pests.Methods in molecular biology (Clifton, N.J.) · 2022Article
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Authors and funding
12 authors at 3 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) can be used for the protection against agricultural pests through the silencing of genes required for pest fitness. To assess the potential of RNAi approaches in the two-spotted spider mite, Tetranychus urticae, we compared 5 methods for the delivery of double-stranded RNA (dsRNA). These methods include mite feeding on either (i) leaves floating on a dsRNA solution, (ii) dsRNA-expressing plants, (iii) artificial diet supplemented with dsRNA, or (iv) dsRNA-coated leaves, and (v) mite soaking in a dsRNA solution. In all cases, the gene targeted for method validation was the Vacuolar-type H+-ATPase (TuVATPase), encoding a constitutively expressed ATP-driven proton pump located in the membrane. Down-regulation of TuVATPase increased mortality and/or reduced fecundity in all methods, but with variable efficiency. The most efficient methods for dsRNA delivery were direct soaking of mites in the dsRNA solution and mite feeding on dsRNA-coated leaves that mimics dsRNA application as a sprayable pesticide. Both resulted in a dark-body phenotype not observed in mites treated with a control dsRNA. Although with lower efficiency, dsRNA designed for TuVATPase silencing and expressed in transgenic Arabidopsis plants impacted the fitness of mites feeding on these plants. RNAi may thus be a valuable strategy to control spider mite populations, either as a sprayable pesticide or through transgenic crops. This comparative methodological study focusing on the induction of RNAi-based gene silencing in T. urticae paves the way for reverse genetics approaches in this model chelicerate system and prepares large-scale systematic RNAi screens as a first step towards the development of specific RNA-based pesticides. Such alternative molecules may help control spider mites that cause significant damages to crops and ornamental plant species, as well as other chelicerates detrimental to agriculture and health.
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