Observational studyPloS one2017
Impact of environmental microbiota on human microbiota of workers in academic mouse research facilities: An observational study.
Observational study in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- WORKbiota: A Systematic Review about the Effects of Occupational Exposure on Microbiota and Workers' Health.International journal of environmental research and public health · 2022Pooled it
- The classroom microbiome and asthma morbidity in children attending 3 inner-city schools.The Journal of allergy and clinical immunology · 2018Trial
- Classroom microbiome signatures of pest management associate with reduced asthma symptoms.The Journal of allergy and clinical immunology · 2026Article
- Article
- The influence of various sample storage conditions and sample bacterial contamination on concentrations of routine biochemical parameters.Journal of medical biochemistry · 2024Article
- Protocol for the air purification for eosinophilic COPD study (APECS): a randomised controlled trial of home air filtration by HEPA.BMJ open · 2024Article
- Rodent models for oral microbiome research: considerations and challenges- a mini review.Frontiers in oral health · 2024Review
- Effects of Unconventional Work and Shift Work on the Human Gut Microbiota and the Potential of Probiotics to Restore Dysbiosis.Nutrients · 2023Review
- Biodiversity Effects on Human Mental Health via Microbiota Alterations.International journal of environmental research and public health · 2022Review
- The Environmental Microbiome, Allergic Disease, and Asthma.The journal of allergy and clinical immunology. In practice · 2022Article
- Differences of the Nasal Microbiome and Mycobiome by Clinical Characteristics of COPD Patients.Chronic obstructive pulmonary diseases (Miami, Fla.) · 2022Article
- Nasopharyngeal Bacterial Microbiota Composition and SARS-CoV-2 IgG Antibody Maintenance in Asymptomatic/Paucisymptomatic Subjects.Frontiers in cellular and infection microbiology · 2022Article
- Antimicrobials from a feline commensal bacterium inhibit skin infection by drug-resistanteLife · 2021Article
- Evidence for Environmental-Human Microbiota Transfer at a Manufacturing Facility with Novel Work-related Respiratory Disease.American journal of respiratory and critical care medicine · 2020Article
- Indoor Microbial Exposures and Chronic Lung Disease: From Microbial Toxins to the Microbiome.Clinics in chest medicine · 2020Review
- Vertical Stratification in Urban Green Space Aerobiomes.Environmental health perspectives · 2020Article
- Environmental Influences of High-Density Agricultural Animal Operation on Human Forearm Skin Microflora.Microorganisms · 2020Article
- Exposure to greenspaces could reduce the high global burden of pain.Environmental research · 2020Review
- Diversity of nasal microbiota and its interaction with surface microbiota among residents in healthcare institutes.Scientific reports · 2019Article
- Impact of occupational exposure on human microbiota.Current opinion in allergy and clinical immunology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
objectivesTo characterize the microbial environment of workers in academic mouse research facilities using endotoxin, 16S qPCR, and 16S amplicon sequencing. To determine whether the work microbiome contributes to the human microbiome of workers.
methodsWe performed area air sampling from the animal rooms, dirty, middle, and setup cage wash locations in four academic mouse research facilities. 10 workers in the dirty cage wash area underwent personal air sampling as well as repeated collection of nasal, oral, and skin samples before and after the work shift. Environmental samples underwent measurement of endotoxin, mouse allergen, bacteria copy number via 16S qPCR, and microbial identification via 16S rDNA sequencing. 16S rDNA sequencing was also performed on human samples before and after the work shift. SourceTracker was used to identify the contribution of the work microbiome to the human microbiome.
resultsMedian endotoxin levels ranged from undetectable to 1.0 EU/m3. Significant differences in mouse allergen levels, bacterial copy number, microbial richness, and microbial community structure were identified between animal, dirty, middle, and setup cage wash locations. Endotoxin levels had only a moderate correlation with microbial composition. Location within a facility was a stronger predictor of microbial community composition (R2 = 0.41, p = 0.002) than facility. The contribution of the work microbiome to the pre-shift human microbiome of workers was estimated to be 0.1 ± 0.1% for the oral microbiome; 3.1 ± 1.9% for the nasal microbiome; and 3.0 ± 1.5% for the skin microbiome.
conclusionsThe microbial environment of academic animal care facilities varies significantly by location rather than facility. Endotoxin is not a proxy for assessment of environmental microbial exposures using 16S qPCR or 16S rDNA sequencing. The work microbiome contributes to the composition of the nasal and skin microbiome of workers; the clinical implications of this observation should be further studied.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.