ArticlePloS one2017
FTIR spectroscopy of whole cells for the monitoring of yeast apoptosis mediated by p53 over-expression and its suppression by Nigella sativa extracts.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 94 citations in OpenAlex.
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- Human P53 Expression in Yeast: Investigating Its Apoptotic Effects.Mini reviews in medicinal chemistry · 2026Review
- Propolis impairs cellular proliferation by promoting oxidative damage and disrupting macromolecular composition in a yeast model.Scientific reports · 2025Article
- Decoding myasthenia gravis: advanced diagnosis with infrared spectroscopy and machine learning.Scientific reports · 2024Article
- Bio-synthesis and characterization of silver nanoparticles from Trichoderma species against cassava root rot disease.Scientific reports · 2024Article
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- Exploring salivary exosomes as early predictors of oral cancer in susceptible tobacco consumers: noninvasive diagnostic and prognostic applications.Journal of cancer research and clinical oncology · 2023Article
- Self-Supported Biopolymeric Films Based on Onion Bulb (Polymers · 2023Article
- Green Synthesis of Characterized Silver Nanoparticle UsingAntibiotics (Basel, Switzerland) · 2023Article
- Classification and Prediction by Pigment Content in Lettuce (Plants (Basel, Switzerland) · 2022Article
- Effect of Antibiotic Amphotericin B Combinations with Selected 1,3,4-Thiadiazole Derivatives on RPTECs in an In Vitro Model.International journal of molecular sciences · 2022Article
- Extracellular vesicles derived from microgreens of Raphanus sativus L. var. caudatus Alef contain bioactive macromolecules and inhibit HCT116 cells proliferation.Scientific reports · 2022Article
- Whole and Purified Aqueous Extracts ofCells · 2022Article
- Managing MMP-2, MMP-9, VEGFR-2, TGFβ-1, and TIMP-1 in NNK-induced lung carcinoma by nonchemical interventions in female rats.Toxicology reports · 2022Article
- Hydroxycinnamic acid derivatives effect on hypercholesterolemia, comparison with ezetimibe: Permeability assays and FTIR spectroscopy on Caco-2 cell line.Current research in pharmacology and drug discovery · 2022Article
- Pulsed Electric Field (PEF) Enhances Iron Uptake by the YeastBiomolecules · 2021Article
- Graphene Oxide-Induced Protein Conformational Change in Nasopharyngeal Carcinoma Cells: A Joint Research on Cytotoxicity and Photon Therapy.Materials (Basel, Switzerland) · 2021Article
- CoronaVac (Sinovac) COVID-19 vaccine-induced molecular changes in healthy human serum by infrared spectroscopy coupled with chemometrics.Turkish journal of biology = Turk biyoloji dergisi · 2021Article
- Embryo-derived teratoma in vitro biological system reveals antitumor and embryotoxic activity of valproate.The FEBS journal · 2020Article
- FTIR and Raman Spectroscopy-Based Biochemical Profiling Reflects Genomic Diversity of ClinicalInternational journal of molecular sciences · 2019Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
p53 over expression in yeast results in cell death with typical markers of apoptosis such as DNA fragmentation and phosphatidylserine externalization. We aimed to substitute/supplement classical fluorescent techniques (TUNEL, Annexin V, ROS detection) usually used to detect biochemical changes occurring during yeast apoptosis mediated by p53 over expression and the effect of anti-apoptotic purified molecules from Nigel (Nigella sativa) extracts on these same yeasts by the label free technique of FTIR spectroscopy. The comparison of the entire IR spectra highlighted clear modifications between apoptotic p53-expressing yeasts and normal ones. More precisely, DNA damage was detected by the decrease of band intensities at 1079 and 1048 cm-1. While phosphatidylserine exposure was followed by the increase of νsCH2 and νasCH2 bands of unsaturated fatty acids that were exhibited at 2855 and 2926 cm-1, and the appearance of the C = O ester functional group band at 1740 cm-1. In a second step, this FTIR approach was used to estimate the effect of a purified fraction of the Nigel extract. The modulation of band intensities specific to DNA and membrane status was in agreement with apoptosis supression in presence of the Nigel extracts. FTIR spectroscopy is thus proven to be a very reliable technique to monitor the apoptotic cell death in yeast and to be used as a means of evaluating the biomolecules effect on yeast survival.
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