ArticleParasitology research2017
Exposure to Anisakis extracts can induce inflammation on in vitro cultured human colonic cells.
Article in Parasitology research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- Anisakiasis: A Decade of Molecular and Diagnostic Advancements (2015-2026).International journal of molecular sciences · 2026Review
- Allergic Anisakiasis: An Integrated Review of Human, Animal and Cellular Evidence.Biomolecules · 2026Review
- Prevalence of Anti-Pathogens (Basel, Switzerland) · 2025Article
- Dual roles ofFrontiers in immunology · 2025Article
- Exploring the exoproteome of the parasitic nematodeFrontiers in immunology · 2025Article
- Anti-Anisakis antibodies in colon cancer patients and their relationship with γδ T-cells.Parasitology research · 2024Article
- Inflammatory Response in Caco-2 Cells Stimulated withPathogens (Basel, Switzerland) · 2022Article
- A miRNAs catalogue from third-stage larvae and extracellular vesicles of Anisakis pegreffii provides new clues for host-parasite interplay.Scientific reports · 2022Article
- Anisakicidal Effects of R (+) Limonene: An Alternative to Freezing Treatment in the Industrial Anchovy Marinating Process.Foods (Basel, Switzerland) · 2022Article
- What Do In Vitro and In Vivo Models Tell Us about Anisakiasis? New Tools Still to Be Explored.Pathogens (Basel, Switzerland) · 2022Review
- Article
- Review
- Anisakis simplex products impair intestinal epithelial barrier function and occludin and zonula occludens-1 localisation in differentiated Caco-2 cells.PLoS neglected tropical diseases · 2020Article
- Exploring tumourigenic potential of the parasite Anisakis: a pilot study.Parasitology research · 2018Article
- Quality assessment ofItalian journal of food safety · 2018Article
- Parasites in Food: From a Neglected Position to an Emerging Issue.Advances in food and nutrition researchReview
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Anisakis spp. is a parasitic nematode whose infective third-stage larvae may be found within the flesh of fish species commonly consumed by humans. Thorough cooking or freezing should render the fish safe for consumption; furthermore, marinating solutions containing biocidal agents might have a significant action against Anisakis larvae. Some studies suggest a relationship between some parasitic infections and development of inflammatory bowel disorders, and Anisakis infection might be a risk factor for stomach or colon cancer. The aim of our study was to investigate if crude extracts (CEs) obtained from Anisakis larvae marinated in a solution with added allyl isothiocyanate (ACE-AITC) and frozen, or from frozen only Anisakis larvae (ACE), can induce an inflammatory effect on in vitro differentiated colonic Caco-2 cells exposed or not to LPS. Caco-2 exposure to the two CEs induced a marked COX-2 expression and potentiated LPS-induced COX-2 overexpression, confirming that substances present in Anisakis larvae can induce an inflammatory response in the intestinal epithelium, possibly also exacerbating the effects of other inflammatory stimuli. ACE induced a marked decrease in caspase-3 activation, while AITC-ACE increased its activation. However, LPS-induced caspase-3 activation appeared lower in cells treated with ACE and with the lower concentration of AITC-ACE. Thus, it is evident that Anisakis CEs may affect various cell pathways crucial not only in the inflammatory process but also in cell growth and death. Thus, CEs obtained from nonviable Anisakis larvae retain or are otherwise provided with noxious properties able to induce a strong inflammation response in intestinal epithelial cells. Furthermore, their influence may persist also following pretreatment with the biocidal agent AITC, indicating that the harmful substances contained in crude extracts from Anisakis larvae are resistant to the thermal or biocidal agent treatments.
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