ArticleThe Analyst2017
Assessing chemotherapeutic effectiveness using a paper-based tumor model.
Article in The Analyst, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Opportunities and challenges for analytical chemists: organ-on-chip devices as new approach methods (NAMs) for identifying potential toxins.The Analyst · 2026Review
- Co-culture of natural killer cells and tumor spheroids on a heterogeneous multilayer paper stack.Journal of Zhejiang University. Science. B · 2024Article
- Article
- A three-zone hypoxia chamber capable of regulating unique oxygen and carbon dioxide partial pressures simultaneously.HardwareX · 2024Article
- Continuous flow delivery system for the perfusion of scaffold-based 3D cultures.Lab on a chip · 2024Article
- Exploring New Dimensions of Tumor Heterogeneity: The Application of Single Cell Analysis to Organoid-Based 3D In Vitro Models.Advanced healthcare materials · 2023Review
- An Automation Workflow for High-Throughput Manufacturing and Analysis of Scaffold-Supported 3D Tissue Arrays.Advanced healthcare materials · 2023Article
- Selecting the appropriate indirect viability assay for 3D paper-based cultures: a data-driven study.The Analyst · 2023Article
- Spatially resolved quantification of drug metabolism and efficacy in 3D paper-based tumor mimics.Analytica chimica acta · 2021Article
- Physiologically relevant oxygen tensions differentially regulate hepatotoxic responses in HepG2 cells.Toxicology in vitro : an international journal published in association with BIBRA · 2021Article
- Unconventional Biomaterials for Cardiovascular Tissue Engineering.Current opinion in biomedical engineering · 2021Article
- Imaging methods to evaluate tumor microenvironment factors affecting nanoparticle drug delivery and antitumor response.Cancer drug resistance (Alhambra, Calif.) · 2021Article
- Tracking the invasion of breast cancer cells in paper-based 3D cultures by OCT motility analysis.Biomedical optics express · 2020Article
- Applications of paper as a support material in biomedical sciences: A decadal review.Sensors international · 2020Review
- Developing a Drug Screening Platform: MALDI-Mass Spectrometry Imaging of Paper-Based Cultures.Analytical chemistry · 2019Article
- Tissue Papers: Leveraging Paper-Based Microfluidics for the Next Generation of 3D Tissue Models.Analytical chemistry · 2019Article
- Developing novel in vitro methods for the risk assessment of developmental and placental toxicants in the environment.Toxicology and applied pharmacology · 2019Review
- Hypoxia differentially regulates estrogen receptor alpha in 2D and 3D culture formats.Archives of biochemistry and biophysics · 2019Article
- Screening Estrogen Receptor Modulators in a Paper-Based Breast Cancer Model.Analytical chemistry · 2018Article
- A three-dimensional engineered heterogeneous tumor model for assessing cellular environment and response.Nature protocols · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
In vitro models for screening new cancer chemotherapeutics often rely on two-dimensional cultures to predict therapeutic potential. Unfortunately, the predictive power of these models is limited, as they fail to recapitulate the complex three-dimensional environments in tumors that promote a chemoresistant phenotype. In this study, we describe the preparation and characterization of paper-based cultures (PBCs) engineered to assess chemotherapeutic effectiveness in three dimensional, diffusion-limited environments. Similar environments are found in poorly vascularized tumors. Monotonic gradients develop across these cultures, which are assembled by stacking cell-laden paper scaffolds to yield thick tissue-like structures, and provide distinct chemical environments for each scaffold. After prolonged incubation, the scaffolds can simply be peeled apart and analyzed. Through fluorescence imaging, we determined that viable and proliferative cell populations were most abundant in scaffolds close to the nutrient-rich medium. By adjusting the cell density, we modulated the spatiotemporal evolution of oxygen gradients across the cultures and correlated these environmental changes with cellular sensitivity to SN-38 exposure. From these results, we showed that differences in the oxygen gradients produced cellular populations with significantly different chemosensitivities. Through this work, we highlight PBCs ability to serve as an analytical model capable of determining chemotherapeutic effectiveness under a range of chemical environments.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.